Tumour growth in mice resistant to diet-induced obesity

Gabi U Dachs, Elisabeth Phillips, Yen Phung, Arron Dyer, Jinny A Willis, Margaret J Currie, Bridget A Robinson

Abstract


Obesity is a chronic disease with associated increases in the incidence, and a reduction in survival, of many cancer types. Obesity results from an imbalance in calorie intake and calorie requirement. This study aimed to investigate the separate effects of high-fat diet and obesity on cancer in an animal model resistant to diet-induced obesity. Male BALB/c mice fed long-term on a high-fat, Western-style diet were implanted with syngeneic CT26 colon adenocarcinoma cells and compared to mice fed normal diet. BALB/c mice on high-fat diet were 10% heavier than mice fed normal diet, with no difference in tumour growth rates or tumour cell proliferation. Subgroups of mice that became obese on high-fat diet, however, showed increased tumour growth rates compared to mice fed normal diet, whereas mice that remained slim showed no difference in tumour growth. Protein arrays identified several adipokines that were expressed at different levels, including serum Tissue Inhibitors of Metallo-Proteinases (TIMP-1) and tumour C-Reactive Protein (CRP). In conclusion, tumour growth was enhanced in mice unable to resist obesity, and adipokine profiles were affected by the animals’ ability to resist obesity.


Keywords


mouse model; BALB/c; syngeneic tumour; Western diet; obesity; colorectal cancer

References


Campbell PT, Newton CC, Dehal AN, Jacobs EJ, Patel AV & Gapstur SM 2012 Impact of body mass index on survival after colorectal cancer diagnosis: the Cancer Prevention Study-II Nutrition Cohort. J Clin Oncol 30 42-52

Crandall DL, Hausman GJ & Kral JG 1997 A review of the microcirculation of adipose tissue: anatomic, metabolic, and angiogenic perspectives. Microcirculation 4 211-232

Dougherty U, Cerasi D, Taylor I, Kocherginsky M, Tekin U, Badal S, Aluri L, Sehdev A, Cerda S, Mustafi R, Delgado J, Joseph L, Zhu H, Hart J, Threadgill D, Fichera A & Bissonnette M 2009 Epidermal growth factor receptor is required for colonic tumor promotion by dietary fat in the azoxymethane/dextran sulfate sodium model: roles of transforming growth factor-{alpha} and PTGS2. Clin Cancer Res 15 6780-6789

Erdelyi I, Levenkova N, Lin EY, Pinto JT, Lipkin M, Quimby FW & Holt PR 2009 Western-style diets induce oxidative stress and dysregulate immune responses in the colon in a mouse model of sporadic colon cancer. J Nutr 139 2072-2078

Fearnside JF, Dumas ME, Rothwell AR, Wilder SP, Cloarec O, Toye A, Blancher C, Holmes E, Tatoud R, Barton RH, Scott J, Nicholson JK & Gauguier D 2008 Phylometabonomic patterns of adaptation to high fat diet feeding in inbred mice. PLoS One 3 e1668

Gerring Z, Pearson JF, Morrin HR, Robinson BA, Harris GC & Walker LC 2015 Phosphohistone H3 outperforms Ki67 as a marker of outcome for breast cancer patients. Histopathology doi: 10.1111/his.12678

Kim EJ, Choi MR, Park H, Kim M, Hong JE, Lee JY, Chun HS, Lee KW & Yoon Park JH 2011 Dietary fat increases solid tumor growth and metastasis of 4T1 murine mammary carcinoma cells and mortality in obesity-resistant BALB/c mice. Breast Cancer Res 13 R78

Kim HY, Kim M, Park HM, Kim J, Kim EJ, Lee CH & Park JH 2014 Lysophospholipid profile in serum and liver by high-fat diet and tumor induction in obesity-resistant BALB/c mice. Nutrition 30 1433-1441

Kim IW, Myung SJ, Do MY, Ryu YM, Kim MJ, Do EJ, Park S, Yoon SM, Ye BD, Byeon JS, Yang SK & Kim JH 2010 Western-style diets induce macrophage infiltration and contribute to colitis-associated carcinogenesis. J Gastroenterol Hepatol 25 1785-1794

Kopitz C, Gerg M, Bandapalli OR, Ister D, Pennington CJ, Hauser S, Flechsig C, Krell HW, Antolovic D, Brew K, Nagase H, Stangl M, von Weyhern CW, Brücher BL, Brand K, Coussens LM, Edwards DR & Krüger A 2007 Tissue inhibitor of metalloproteinases-1 promotes liver metastasis by induction of hepatocyte growth factor signaling. Cancer Res 67 8615-8623

Lamas B, Nachat-Kappes R, Goncalves-Mendes N, Mishellany F, Rossary A, Vasson MP & Farges MC 2015 Dietary fat without body weight gain increases in vivo MCF-7 human breast cancer cell growth and decreases natural killer cell cytotoxicity. Mol Carcinog 54 58-71

Meijer J, Ogink J & Roos E 2008 Effect of the chemokine receptor CXCR7 on proliferation of carcinoma cells in vitro and in vivo. Br J Cancer 99 1493-1501

Meyerhardt JA, Tepper JE, Niedzwiecki D, Hollis DR, McCollum AD, Brady D, O'Connell MJ, Mayer RJ, Cummings B, Willett C, Macdonald JS, Benson AB 3rd & Fuchs CS 2004 Impact of body mass index on outcomes and treatment-related toxicity in patients with stage II and III rectal cancer: findings from Intergroup Trial 0114. J Clin Oncol 22 648-657

Montgomery MK, Hallahan NL, Brown SH, Liu M, Mitchell TW, Cooney GJ & Turner N 2013 Mouse strain-dependent variation in obesity and glucose homeostasis in response to high-fat feeding. Diabetologia 56 1129-1139

Nanri A, Moore MA & Kono S 2007 Impact of C-reactive protein on disease risk and its relation to dietary factors. Asian Pac J Cancer Prev 8 167-177

Newmark HL, Yang K, Lipkin M, Kopelovich L, Liu Y, Fan K & Shinozaki H 2001 A Western-style diet induces benign and malignant neoplasms in the colon of normal C57Bl/6 mice. Carcinogenesis 22 1871-1875

Nishikawa S, Sugimoto J, Okada M, Sakairi T & Takagi S 2012 Gene expression in livers of BALB/C and C57BL/6J mice fed a high-fat diet. Toxicol Pathol 40 71-82

Nishikawa S, Yasoshima A, Doi K, Nakayama H & Uetsuka K 2007 Involvement of sex, strain and age factors in high fat diet-induced obesity in C57BL/6J and BALB/cA mice. Exp Anim 56 263-272

Parekh N, Chandran U & Bandera EV 2012 Obesity in cancer survival. Annu Rev Nutr 32 311-342

Park H, Kim M, Kwon GT, Lim do Y, Yu R, Sung MK, Lee KW, Daily JW 3rd & Park JH 2012 A high-fat diet increases angiogenesis, solid tumor growth, and lung metastasis of CT26 colon cancer cells in obesity-resistant BALB/c mice. Mol Carcinog 51 869-880

Park J, Morley TS, Kim M, Clegg DJ & Scherer PE 2014 Obesity and cancer--mechanisms underlying tumour progression and recurrence. Nat Rev Endocrinol 10 455-465

Richter F, Newmark HL, Richter A, Leung D & Lipkin M 1995 Inhibition of Western-diet induced hyperproliferation and hyperplasia in mouse colon by two sources of calcium. Carcinogenesis 16 2685-2689

Risio M, Lipkin M, Newmark H, Yang K, Rossini FP, Steele VE, Boone CW & Kelloff GJ 1996 Apoptosis, cell replication, and Western-style diet-induced tumorigenesis in mouse colon. Cancer Res 56 4910-4916

Schelter F, Halbgewachs B, Bäumler P, Neu C, Görlach A, Schrötzlmair F & Krüger A 2011 Tissue inhibitor of metalloproteinases-1-induced scattered liver metastasis is mediated by hypoxia-inducible factor-1α. Clin Exp Metastasis 28 91-99

Waller-Evans H, Hue C, Fearnside J, Rothwell AR, Lockstone HE, Caldérari S, Wilder SP, Cazier JB, Scott J & Gauguier D 2013Nutrigenomics of high fat diet induced obesity in mice suggests relationships between susceptibility to fatty liver disease and the proteasome. PLoS One 8 e82825

Workman P, Aboagye EO, Balkwill F, Balmain A, Bruder G, Chaplin DJ, Double JA, Everitt J, Farningham DA, Glennie MJ, Kelland LR, Robinson V, Stratford IJ, Tozer GM, Watson S, Wedge SR, Eccles SA & Committee of the National Cancer Research Institute 2010 Guidelines for the welfare and use of animals in cancer research. Br J Cancer 102 1555-1577

World Cancer Research Fund 2007 Retrieved on July 15, 2015 from http://www.dietandcancerreport.org/cancer_resource_center/downloads/Second_Expert_Report_full.pdf

Xue L, Yang K, Newmark H, Leung D & Lipkin M 1996 Epithelial cell hyperproliferation induced in the exocrine pancreas of mice by a western-style diet. J Natl Cancer Inst 88 1586-1590


Full Text: PDF

Refbacks

  • There are currently no refbacks.


Copyright © 2017 Lorem Ipsum Press