2024-03-29T06:59:06Z
http://www.jmolbiochem.com/index.php/JmolBiochem/oai
oai:ojs.jmolbiochem.com:article/24
2012-02-20T17:57:43Z
JmolBiochem:ED
"120215 2012 eng "
dc
The Shape of Science
Vlachakis, Dimitrios; Biomedical Research Foundation, Academy of Athens, Greece
Champeris Tsaniras, Spyridon; Department of Physiology, Medical School, University of Patras, Rio, Patras, Greece
Lorem Ipsum Press
2012-02-15 17:34:13
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/24
Journal of Molecular Biochemistry; Vol 1, No 1 (2012)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/3
2012-02-20T17:57:43Z
JmolBiochem:ART
"120215 2012 eng "
dc
Leishmania Donovani Cell Surface Sialoglycans Regulate Susceptibility for Siglec Mediated Macrophage Invasion and Parasite Survival
Karmakar, Subir; Indian Institute of Chemical Biology
Bhaumik, Siddhartha K; Indian Institute of Chemical Biology
Paul, Joydeep; Indian Institute of Chemical Biology
De, Tripti; Indian Institute of Chemical Biology
Leishmania donovani; sialoglycans; macrophage; sialoadhesine; p38 MAPK; ERK; JNK
<p>Glycoconjugates play a pivotal role in the survival of <em>Leishmania</em> parasites in destructive surroundings. An important constituent present on many glycoconjugates is sialic acid. By virtue of their peripheral position on oligosaccharide chains of glycoconjugates, sialic acids are well suited as molecular determinants of specific biological processes, including the interaction of pathogenic microorganisms with sialylated cellular receptors. Differences in a2,3- and a2,6-sialoglycan patterns detected in clonal virulent <em>Leishmania donovani</em> promastigotes, correlated with the level of a2,3- and a2,6-sialyltransferase activity present in these parasites. The role of macrophage sialic acid-receptors in uptake and survival of <em>L.donovani</em> was studied in the murine macrophage cell line raw 264.7. Macrophage invasion was dependent on the binding to Siglec-1, while suppression of MAPK signaling was mediated through Siglec-5. Sialic acid removal by neuraminidase treatment reduced parasite infectivity. The presence of trypsin resistant sialic acid residues in the neuraminidase treated parasites grown in a serum free medium in presence of sialoglycoconjugates indicated that the parasites could salvage sialic acid from exogenous sialoglycans and reutilize it for <em>de novo</em> glycoprotein sialylation in <em>L.donovani</em> parasites. Thus, our results demonstrate the involvement of sialoglycans in the invasion as well as the survival process of <em>L.donovani</em> parasites.</p>
Lorem Ipsum Press
CSIR
2012-02-15 17:34:13
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/3
Journal of Molecular Biochemistry; Vol 1, No 1 (2012)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/9
2012-02-20T17:57:43Z
JmolBiochem:ART
"120215 2012 eng "
dc
Therapeutic efficacy of an oncolytic adenovirus containing RGD ligand in minor capsid protein IX and Fiber, Δ24DoubleRGD, in an ovarian cancer model
Gamble, Lena J; Division of Human Gene Therapy, Departments of Medicine, Pathology, Surgery, Obstetrics and Gynecology; University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Ugai, Hideyo; Division of Human Gene Therapy, Departments of Medicine, Pathology, Surgery, Obstetrics and Gynecology; University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Wang, Minghui; Division of Human Gene Therapy, Departments of Medicine, Pathology, Surgery, Obstetrics and Gynecology; University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Borovjagin, Anton V; School of Dentistry, Institute of Oral Health Research; University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Matthews, Qiana L; Division of Human Gene Therapy, Departments of Medicine, Pathology, Surgery, Obstetrics and Gynecology; the Gene Therapy Center; Center for AIDS Research; Division of Infectious Diseases; University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Conditionally replicative adenoviruses (CRAds); Ovarian Cancer; arginine-glycine-aspartate (RGD) motif; capsid-modification; anti-cancer therapy
<p>Ovarian cancer is the leading cause of gynecological disease death despite advances in medicine. Therefore, novel strategies are required for ovarian cancer therapy. Conditionally replicative adenoviruses (CRAds), genetically modified as anti-cancer therapeutics, are one of the most attractive candidate agents for cancer therapy. However, a paucity of coxsackie B virus and adenovirus receptor (CAR) expression on the surface of ovarian cancer cells has impeded treatment of ovarian cancer using this approach.<br />This study sought to engineer a CRAd with enhanced oncolytic ability in ovarian cancer cells, “Δ24DoubleRGD.” Δ24DoubleRGD carries an arginine-glycine-aspartate (RGD) motif incorporated into both fiber and capsid protein IX (pIX) and its oncolytic efficacy was evaluated in ovarian cancer. In vitro analysis of cell viability showed that infection of ovarian cancer cells with Δ24DoubleRGD leads to increased cell killing relative to the control CRAds. Data from this study suggested that not only an increase in number of RGD motifs on the CRAd capsid, but also a change in the repertoir of targeted integrins could lead to enhanced oncolytic potency of Δ24DoubleRGD in ovarian cancer cells <em>in vitro</em>. In an intraperitoneal model of ovarian cancer, mice injected with Δ24DoubleRGD showed, however, a similar survival rate as mice treated with control CRAds.</p>
Lorem Ipsum Press
NIH
2012-02-15 17:34:13
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/9
Journal of Molecular Biochemistry; Vol 1, No 1 (2012)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/8
2012-02-20T17:57:43Z
JmolBiochem:ART
"120215 2012 eng "
dc
N2-Phenyl-9-(hydroxyalkyl)guanines and related compounds are substrates for Herpes simplex thymidine kinases
Lossani, Andrea; Istituto di Genetica Molecolare
Savi, Lida; Istituto di Genetica Molecolare
Manikowski, Andrzej; GLSynthesis Inc.
Maioli, Andrew; GLSynthesis Inc.
Gambino, Joseph; GLSynthesis Inc.
Focher, Federico; Istituto di Genetica Molecolare
Spadari, Silvio; Istituto di Genetica Molecolare
Wright, George E; GLSynthesis Inc.
Herpesvirus; phosphorylation; substrate; antiviral; reactivation
<div><div class="section"><div class="section"><div class="section"><div class="layoutArea"><div class="column"><p><span>Herpes simplex virus (HSV) types 1 and 2 thymidine kinases (TK) are responsible for phosphorylation of antiherpes acyclonucleosides such as acyclovir (ACV) and 9-(4-hydroxybutyl)guanine (HBG). Related compounds, the N<sup>2</sup>-phenyl-9-(hydroxyalkyl)guanines, are devoid of direct antiviral activity, but potently inhibit the viral TKs and block viral reactivation from latency </span><span><em>in vivo</em>. </span><span>The similarity in structure between the acyclonucleosides and TK inhibitors raised the question of the relevance of phosphorylation of certain of the latter analogs in their mechanisms of action. Using recombinant TKs and HPLC analysis of reaction mixtures, we report that the lead TK inhibitor N<sup>2</sup>-phenyl-9-(4-hydroxybutyl)guanine (HBPG) and its pentyl ho</span><span>molog (HPnPG) are excellent substrates for the enzymes, approaching the efficiency with which the natural substrate thymidine is phosphorylated, and significantly better than ACV or HBG. Other 9-hydroxyalkyl congeners are substrates for the enzymes, but with much poorer efficiency. HBPG triphosphate was a poor inhibitor of HSV DNA polymerase, the target of acyclonucleoside triphosphates, suggesting that phosphorylation of HBPG is not important in its mechanism of blocking viral reactivation </span><span><em>in vivo</em>. </span><span>The fact that HBPG is an efficient substrate is consistent, however, with its binding mode based both on molecular modeling studies and x-ray structure of the HBPG:TK complex.</span></p></div></div></div></div></div></div>
Lorem Ipsum Press
NIH, FIRB
2012-02-15 17:34:13
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/8
Journal of Molecular Biochemistry; Vol 1, No 1 (2012)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/18
2012-06-19T05:49:22Z
JmolBiochem:REV
"120215 2012 eng "
dc
Modulation of monocyte/macrophage-derived cytokine and chemokine profile by persistent Hepatitis C virus (HCV) infection leads to chronic inflammation
Kochlios, Emmanouil; Hellenic Pasteur Institute
Foka, Pelagia; Hellenic Pasteur Institute
Mavromara, Penelope; Hellenic Pasteur Institute
HCV; monocytes; macrophages; cytokines; chemokines; inflammation; hepatocellular carcinoma; lymphotropism
<p>HCV infection presents a major public health problem, with more than 170 million people infected worldwide. Chronicity and persistence of infection constitute the hallmark of the disease. Although HCV is a hepatotropic virus, subsets of immune cells have been found to be permissive to infection and viral replication. Peripheral blood monocytes, attracted to the site of infection and differentiated into macrophages, and resident hepatic macrophages, known as Kupffer cells, are important mediators of innate immunity, through production of several chemokines and cytokines in addition to their phagocytic activity. HCV proteins have been shown to modulate the cytokine and chemokine production profile of monocytes/macrophages, as it is suggested by both <em>in vitro</em> and clinical studies. This modified expression profile appears crucial for the establishment of aberrant inflammation that leads to liver cirrhosis and hepatocellular carcinoma.</p>
Lorem Ipsum Press
2012-02-15 17:34:13
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/18
Journal of Molecular Biochemistry; Vol 1, No 1 (2012)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/19
2012-02-20T17:57:43Z
JmolBiochem:ART
"120215 2012 eng "
dc
Prediction of Peptide Binding to Major Histocompatibility II Receptors with Molecular Mechanics and Semi-Empirical Quantum Mechanics Methods
Aldulaijan, Sarah; School of Chemistry, Cardiff University
Platts, James A; School of Chemistry, Cardiff University
major histocompatibility complex; molecular mechanics; quantum mechanics; density functional theory
<p>Methods for prediction of the binding of peptides to major histocompatibility complex (MHC) II receptors are examined, using literature values of IC<sub>50</sub> as a benchmark. Two sets of IC<sub>50</sub> data for closely structurally related peptides based on hen egg lysozyme (HEL) and myelin basic protein (MBP) are reported first. This shows that methods based on both molecular mechanics and semi-empirical quantum mechanics can predict binding with good-to-reasonable accuracy, as long as a suitable method for estimation of solvation effects is included. A more diverse set of 22 peptides bound to HLA-DR1 provides a tougher test of such methods, especially since no crystal structure is available for these peptide-MHC complexes. We therefore use sequence based methods such as SYFPEITHI and SVMHC to generate possible binding poses, using a consensus approach to determine the most likely anchor residues, which are then mapped onto the crystal structure of an unrelated peptide bound to the same receptor. This analysis shows that the MM/GBVI method performs particularly well, as does the AMBER94 forcefield with Born solvation model. Indeed, MM/GBVI can be used as an alternative to sequence based methods in generating binding poses, leading to still better accuracy.</p>
Lorem Ipsum Press
Leverhulme Trust
2012-02-15 17:34:13
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/19
Journal of Molecular Biochemistry; Vol 1, No 1 (2012)
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Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/70
2012-06-16T12:02:23Z
JmolBiochem:ED
"120616 2012 eng "
dc
An introduction to M-theory and its application in biology
Vlachakis, Dimitrios
Champeris Tsaniras, Spyridon
M-theory; string theory; Schrödinger equation; quantum mechanics; molecular modelling
Lorem Ipsum Press
2012-06-16 12:06:38
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/70
Journal of Molecular Biochemistry; Vol 1, No 2 (2012)
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Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/15
2012-06-16T12:02:23Z
JmolBiochem:ART
"120616 2012 eng "
dc
Thianthrene is a novel inhibitor of Leishmania donovani pteridine reductase 1 (PTR1)
Kaur, Jaspreet
Dube, Divya
Ramachandran, Ravishankar
Singh, Prashant
Singh, Neeloo
antileishmanial agent; pteridine reductase 1; leishmania donovani; molecular modeling; in silico docking; flow cytometry; thianthrene
<p>Pteridine reductase 1 (PTR1) from <em>Leishmania donovani</em> is a short chain reductase that catalyses the NADPH-dependent reduction of folates and pterins. It has gained attention as a therapeutic target because it acts as a metabolic bypass for dihydrofolate reductase (DHFR) targeting drugs and is thought to be responsible for the failure of conventional therapies against the trypanosomatids. In the present study, we report the identification of thianthrene as a potent inhibitor of <em>L. donovani</em> PTR1 (<em>Ld</em>PTR1) based on both structure-based virtual screening and experimental verification. Thianthrene displayed uncompetitive mixed type inhibition in a recombinant enzyme inhibition assay. In addition, cell based assays and flow cytometry showed that the intracellular amastigotes were inhibited by thianthrene <em>in vitro</em>. The results of our study could be considered for the development of novel therapeutics based on PTR1 inhibition.</p>
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Department of Biotechnology, New Delhi, India and Council of Scientific and Industrial Research
2012-06-16 12:06:38
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/15
Journal of Molecular Biochemistry; Vol 1, No 2 (2012)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/25
2012-06-16T12:02:23Z
JmolBiochem:ART
"120616 2012 eng "
dc
Probing the GnRH receptor agonist binding site identifies methylated triptorelin as a new anti-proliferative agent
Morgan, Kevin; MRC Human Reproductive Sciences Unit
Leighton, Samuel P; MRC HRSU
Millar, Robert P; MRC HRSU
GnRH agonist analogues, D-amino acid residue, anti-proliferation, 5-Methyl-DL-Trp6 GnRH, binding affinity, EC50, inositol phosphate, HEK293[SCL60], prostate, WPE-1-NB26, Triptorelin, methylated Triptorelin, Leuprolide, GnRH-I, GnRH-II
<p>D-amino acid substitutions at Glycine postion-6 in GnRH-I decapeptide can possess super-agonist activity and enhanced <em>in vivo</em> pharmacokinetics. Agonists elicit growth-inhibition in tumorigenic cells expressing the GnRH receptor above threshold levels. However, new agonists with modified properties are required to improve the anti-proliferative range. Effects of residue substitutions and methylations on tumourigenic HEK293<sub>[SCL60]</sub> and WPE-1-NB26-3 prostate cells expressing the rat GnRH receptor were compared. Peptides were ranked according to receptor binding affinity, induction of inositol phosphate production and cell growth-inhibition. Analogues possessing D-Trp<sup>6</sup> (including Triptorelin), D-Leu<sup>6</sup> (including Leuprolide), D-Ala<sup>6</sup>, D-Lys<sup>6</sup>, or D-Arg<sup>6</sup> exhibited agonist and anti-proliferative activity. Residues His<sup>5</sup> or His<sup>5</sup>,Trp<sup>7</sup>,Tyr<sup>8</sup>, corresponding to residues found in GnRH-II , were tolerated, with retention of sub-nanomolar/low nanomolar binding affinities and EC<sub>50</sub>s for receptor activation and IC<sub>50</sub>s for cell growth-inhibition. His<sup>5</sup>D-Arg<sup>6</sup>-GnRH-I exhibited reduced binding affinity and potency, effective in the mid-nanomolar range. However, all GnRH-II-like analogues were less potent than Triptorelin. By comparison, three methylated-Trp<sup>6</sup> Triptorelin variants showed differential binding, receptor activation and anti-proliferation potency. Significantly, 5-Methyl-DL-Trp<sup>6</sup>-Triptorelin was equipotent to triptorelin. Subsequent studies should determine whether pharmacologically enhanced derivatives of Triptorelin can be developed by further alkylations, without substitutions or cleavable cytotoxic adducts, to improve the extent of growth-inhibition of tumour cells expressing the GnRH receptor.</p>
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Medical Research Council UK
2012-06-16 12:06:38
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/25
Journal of Molecular Biochemistry; Vol 1, No 2 (2012)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/27
2012-06-19T05:50:24Z
JmolBiochem:REV
"120616 2012 eng "
dc
Vertebrate scavenger receptor class B member 2 (SCARB2): comparative studies of a major lysosomal membrane glycoprotein
Holmes, Roger Stephen; Griffith University
Vertebrates; SCARB2; comparative proteomics; comparative genomics; evolution; thrombospondin receptor
<p>Scavenger receptor class B member 2 (SCARB2) (also LIMP-2, CD36L2 or LGP85) is a major lysosomal membrane glycoprotein involved in endosomal and lysosomal biogenesis and maintenance. SCARB2 acts as a receptor for the lysosomal mannose-6-phosphate independent targeting of β-glucuronidase and enterovirus 71 and influences Parkinson’s disease and epilepsy. Genetic deficiency of this protein causes deafness and peripheral neuropathy in mice as well as myoclonic epilepsy and nephrotic syndrome in humans. Comparative SCARB2 amino acid sequences and structures and <em>SCARB2</em> gene locations were examined using data from several vertebrate genome projects. Vertebrate SCARB2 sequences shared 43-100% identity as compared with 30-36% sequence identities with other CD36-like superfamily members, SCARB1 and CD36. At least 10 N-glycosylation sites were conserved among most vertebrate SCARB2 proteins examined. Sequence alignments, key amino acid residues and conserved predicted secondary structures were examined, including cytoplasmic, transmembrane and external lysosomal membrane sequences: cysteine disulfide residues, thrombospondin (THP1) binding sites and 16 proline and 20 glycine conserved residues, which may contribute to short loop formation within the exomembrane SCARB2 sequences. Vertebrate <em>SCARB2 </em>genes contained 12 coding exons. The human <em>SCARB2</em> gene contained a CpG island (CpG100), ten microRNA-binding sites and several transcription factor binding sites (including PPARA) which may contribute to a higher level (2.4 times average) of gene expression. Phylogenetic analyses examined the relationships and potential evolutionary origins of the vertebrate <em>SCARB2 </em>gene with vertebrate <em>SCARB1</em> and <em>CD36</em> genes. These suggested that <em>SCARB2</em> originated from duplications of the <em>CD36</em> gene in an ancestral genome forming three vertebrate <em>CD36</em> gene family members: <em>SCARB1</em>, <em>SCARB2</em> and <em>CD36</em>.</p>
Lorem Ipsum Press
2012-06-16 12:06:38
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/27
Journal of Molecular Biochemistry; Vol 1, No 2 (2012)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/27/201
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/43
2012-06-16T12:02:23Z
JmolBiochem:ART
"120616 2012 eng "
dc
Enhanced fold recognition using efficient short fragment clustering
Krissinel, Evgeny; CCP4, Research Complex at Harwell
structure alignment; protein fold recognition; structure superposition; GESAMT
<p lang="en-US">The main structure aligner in the CCP4 Software Suite, SSM (Secondary Structure Matching) has a limited applicability on the intermediate stages of the structure solution process, when the secondary structure cannot be reliably computed due to structural incompleteness or a fragmented mainchain. In this study, we describe a new algorithm for the alignment and comparison of protein structures in CCP4, which was designed to overcome SSM's limitations but retain its quality and speed. The new algorithm, named GESAMT (General Efficient Structural Alignment of Macromolecular Targets), employs the old idea of deriving the global structure similarity from a promising set of locally similar short fragments, but uses a few technical solutions that make it considerably faster. A comparative sensitivity and selectivity analysis revealed an unexpected significant improvement in the fold recognition properties of the new algorithm, which also makes it useful for applications in the structural bioinformatics domain. The new tool is included in the CCP4 Software Suite starting from version 6.3.</p>
Lorem Ipsum Press
Collaborative Computational Project No 4 in Protein Crystallography (CCP4)
2012-06-16 12:06:38
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/43
Journal of Molecular Biochemistry; Vol 1, No 2 (2012)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/53
2012-06-16T12:02:23Z
JmolBiochem:ART
"120616 2012 eng "
dc
Evaluation of auto-antibody serum biomarkers for breast cancer screening and in silico analysis of sero-reactive proteins
Syed, Parvez; Austrian Institute of Technology
Vierlinger, Klemens
Kriegner, Albert
Sergelen, Khulan
Luna-Coronell, Johana
Rappaport-Fürhauser, Christine
Nöhammer, Christa
Singer, Christian F
Weinhäusel, Andreas
Auto-antibody biomarker; breast cancer; protein microarray; tumour-associated antigens
<p>Aberrantly expressed proteins in tumours evoke an immunological response. These immunogenic proteins can serve as potential biomarkers for the early diagnosis of cancers. In this study, we performed a candidate marker screen on macroarrays containing 38,016 human proteins, derived from a human fetal-brain expression library, with the pools of sera from breast cancer patients (1 pool of benign samples, 3 pools of ductal carcinoma and 2 pools of lobular carcinoma) and 1 pool of sera from healthy women. A panel of 642 sero-reactive clones were deduced from these macroarray experiments which include 284 in-frame clones. Over-representation analyses of the sero-reactive in-frame clones enabled the identification of the sets of genes over-expressed in various pathways of the functional categories (KEGG, Transpath, Pfam and GO). Protein microarrays, generated using the His-tag proteins derived from the macroarray experiments, were used to evaluate the sera from breast cancer patients (24 malignant, 16 benign) and 20 control individuals. Using the PAM algorithm we elucidated a panel of 50 clones which enabled the correct classification prediction of 93% of the breast-nodule positive group (benign & malignant) sera from healthy individuals’ sera with 100% sensitivity and 85% specificity. This was followed by over-representation analysis of the significant clones derived from the class prediction.</p>
Lorem Ipsum Press
Jubiläumsfonds der Österreichischen National bank, Austria
2012-06-16 12:06:38
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/53
Journal of Molecular Biochemistry; Vol 1, No 2 (2012)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/53/205
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/54
2012-06-16T12:02:23Z
JmolBiochem:ART
"120616 2012 eng "
dc
In silico design and performance of peptide microarrays for breast cancer tumour-auto-antibody testing
Syed, Parvez; Austrian Institute of Technology
Gyurján, István
Kriegner, Albert
Vierlinger, Klemens
Singer, Christian F.
Rappaport-Fürhauser, Christine
Zerweck, Johannes
Söllner, Johannes
Weinhäusel, Andreas
Peptide microarrays, auto-antibody, protein microarray, antigenic peptide design, Antigenic motif enrichment
The simplicity and potential of minimally invasive testing using sera from patients makes auto-antibody based biomarkers a very promising tool for use in cancer diagnostics. Protein microarrays have been used for the identification of such auto-antibody signatures. Because high throughput protein expression and purification is laborious, synthetic peptides might be a good alternative for microarray generation and multiplexed analyses.<br /> In this study, we designed 1185 antigenic peptides, deduced from proteins expressed by 642 cDNA expression clones found to be sero-reactive in both breast tumour patients and controls. The sero-reactive proteins and the corresponding peptides were used for the production of protein and peptide microarrays. Serum samples from females with benign and malignant breast tumours and healthy control sera (<em>n</em>=16 per group) were then analysed. Correct classification of the serum samples on peptide microarrays were 78% for discrimination of ‘malignant versus healthy controls’, 72% for ‘benign versus malignant’ and 94% for ‘benign versus controls’. On protein arrays, correct classification for these contrasts was 69%, 59% and 59%, respectively.<br /> The over-representation analysis of the classifiers derived from class prediction showed enrichment of genes associated with ribosomes, spliceosomes, endocytosis and the pentose phosphate pathway. Sequence analyses of the peptides with the highest sero-reactivity demonstrated enrichment of the zinc-finger domain. Peptides’ sero-reactivities were found negatively correlated with hydrophobicity and positively correlated with positive charge, high inter-residue protein contact energies and a secondary structure propensity bias. This study hints at the possibility of using <em>in silico</em> designed antigenic peptide microarrays as an alternative to protein microarrays for the improvement of tumour auto-antibody based diagnostics.<br /><br />
Lorem Ipsum Press
Jubiläumsfonds der Österreichischen National Bank and Vienna Science and Technology Fund
2012-06-16 12:06:38
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/54
Journal of Molecular Biochemistry; Vol 1, No 2 (2012)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/54/208
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/39
2012-10-21T03:53:39Z
JmolBiochem:ART
"121018 2012 eng "
dc
A syntenic coding region for vitelline membrane proteins in four lepidopteran insects
Xu, Yunmin; State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Zou, Ziliang; State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Zha, Xingfu; State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Xiang, Zhonghuai; State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
He, Ningjia; State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China
Lepidoptera; Microsynteny; Vitelline membrane; VM domain; VMP
<p>The vitelline membrane is the inner layer of the eggshell, but the genomic information available for vitelline membrane proteins (VMPs) in Lepidoptera is limited. In the present study, we identified a syntenic coding region for VMPs in four lepidopteran genomes (<em>Bombyx mori</em>, <em>Manduca sexta</em>, <em>Danaus plexippus</em> and <em>Heliconius melpomene</em>) and four putative VMP coding genes located within it. RT-PCR results showed Bombyx VMP coding genes expressed prior to the early choriogenesis stage in follicles. Alignment analyses revealed that the vitelline membrane domain was shared between Lepidoptera and Diptera. However, the third cysteine residue conserved in dipteran VMPs was absent in those of Lepidoptera. In addition, another conserved region was identified in lepidopteran VMPs.</p>
Lorem Ipsum Press
National Basic Research Program of China, Science Fund for Distinguished Young Scholars of Chongqing, Fund for Doctor in Southwest University
2012-10-21 03:53:39
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/39
Journal of Molecular Biochemistry; Vol 1, No 3
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/39/281
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/86
2012-10-21T03:53:39Z
JmolBiochem:ED
"121018 2012 eng "
dc
Current viral infections and epidemics of flaviviridae; lots of grief but also some hope
Vlachakis, Dimitrios
Champeris Tsaniras, Spyridon
Kossida, Sophia; Biomedical Research Foundation, Academy of Athens, Athens, Greece
flaviridiae; viral helicase; nucleoside analog; antiviral agent
<p><em>Flaviviridae</em> is a family of RNA viruses that includes numerous important human and animal pathogens. Recent studies on subgenomic flaviviridae replicons have revealed that the non-structural (NS) proteins, which are encoded by the C-terminal part of the polyprotein, play a crucial role in viral RNA replication. Accordingly, these proteins are assumed to form replication complexes in conjunction with genomic RNA and possibly with other cellular factors. One the most important non-structural enzymes that plays a key role in the life cycle of flaviviridae viruses is the viral helicase. Sequence alignments of the viral helicases from this family identified several conserved sequence motifs that are important for biological functions. Herein, an effort is made to summarize the current epidemics associated with the flaviviridae family worldwide, the potential of helicase enzymes as a promising pharmacological target and the use of nucleoside analogs as simple, efficient and rather versatile antiviral agents.</p>
Lorem Ipsum Press
2012-10-21 03:53:39
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/86
Journal of Molecular Biochemistry; Vol 1, No 3
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/48
2012-10-21T05:22:15Z
JmolBiochem:ART
"121018 2012 eng "
dc
Crystal structural studies of ethyl-5-(4-chlorophenylsulfonyl)-4-hydroxy-2, 6-dip-tolyl-1, 2, 5, 6-tetrahydropyridine-3-carboxylate and diethyl 4-hydroxy-2-(4-nitrophenyl)-5-(phenylsulfonyl)-6-(phenylsulfonylmethyl)cyclohexa-3,6-diene-1,3-dicarboxylate
Suresh, J.; Assistant Professor
The Madura College
Harikrishnan, P.S.; Department of Chemistry, The Madura College, Madurai, 625 011, India
Vishnu Priya, R.; School of Chemistry, Madurai Kamaraj University, Madurai, 625 021, India
Perumal, S.; School of Chemistry, Madurai Kamaraj University, Madurai, 625 021, India
Crystal structure; conformation; coordination interactions; hydrogen bond
<p>The crystal structures of trans,trans-Ethyl-5-(4-chlorophenylsulfonyl)-4-hydroxy-2,6-dip-tolyl-1,2,5,6-tetrahydropyridine-3-carboxylate (Ia) and cis-Diethyl 4-hydroxy-2-(4-nitrophenyl)-5-(phenylsulfonyl)-6-(phenylsulfonylmethyl)cyclohexa-3,6-diene-1,3-dicarboxylate (Ib) were elucidated by single crystal X ray diffraction. Compound (Ia) C<sub>28</sub>H<sub>28</sub>Cl NO<sub>5</sub>S, crystallizes in the monoclinic system, space group P 2<sub>1</sub>/c, with a = 9.4530(7) Å, b = 25.366(2) Å, c = 11.4353(8) Å, β = 103.092(7)°, V = 2670 (3) Å<sup>3</sup> and Z = 4. The compound (Ib), C<sub>31</sub>H<sub>29</sub>N<sub>1</sub>O<sub>11</sub>S<sub>2</sub>, crystallizes in the monoclinic system, space group P 2<sub>1</sub>/c, with a = 21.179(4) Å, b = 8.4998(16) Å, c = 17.347(3) Å, β = 102.563(3)°, V = 3048.0(10) Å<sup>3</sup> and Z = 4. The central piperidine ring of compound (Ia) adopts the sofa conformation and the central cyclohexadiene ring of compound (Ib) adopts the boat conformation. Details of the compounds preparation, crystal structures and hydrogen bonding interactions of the compounds are discussed.</p>
Lorem Ipsum Press
2012-10-21 03:53:39
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/48
Journal of Molecular Biochemistry; Vol 1, No 3
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/48/221
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/48/222
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/56
2012-10-21T03:53:39Z
JmolBiochem:ART
"121018 2012 eng "
dc
Three-dimensional structure-activity relationship modeling of cross-reactivities of a polyclonal antibody against pyrene by comparative molecular field analysis
Li, Yi; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China
Liu, Yuanyuan; Department of Chemical and Pharmaceutical Engineering, Southeast University Chengxian College, Nanjing 210088, PR China
Chen, Hongyan; College of Science, Nanjing University of Technology, Nanjing 210009, PR China
Wei, Ping; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China
Li, Fangshi; College of Science, Nanjing University of Technology, Nanjing 210009, PR China
Polycyclic aromatic hydrocarbons; Immunoassay; Cross-Reactivity; Quantitative structure-activity relationship; Comparative molecular field analysis
<p>Immunoassays have been regarded as a possible alternative or supplement for measuring polycyclic aromatic hydrocarbons (PAHs) in the environment. Since there are too many potential cross-reactants for PAH immunoassays, it is difficult to determine all the cross-reactivities (CRs) by experimental tests. In this study, the quantitative structure-activity relationship (QSAR) technique, comparative molecular field analysis (CoMFA), was applied to predict the CRs of a polyclonal antibody against pyrene. The CoMFA model developed shows that the CRs of the compounds are correlated to their 3D structure (n = 14, q<sup>2</sup>=0.527, r<sup>2</sup>=0.944, and Standard Error (SE) = 0.22478). The contributions of the steric and electrostatic fields to CRs are 95 and 5%, respectively. The results of the correlation predicted the CRs with actual CRs of randomly selected test compounds and showed that the developed model has good prediction ability. The QSAR model has been applied to predict the CRs of other 17 PAHs. The 3D-QSAR model and its respective contour plot could be useful tools to further understand the molecular nature of antibody-antigen interactions.</p>
Lorem Ipsum Press
2012-10-21 03:53:39
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/56
Journal of Molecular Biochemistry; Vol 1, No 3
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/57
2012-10-21T03:53:39Z
JmolBiochem:ART
"121018 2012 eng "
dc
Residue conservation and dimer-interface analysis of olfactory receptor molecular models
Harini, Krishnan; National Centre for Biological Sciences (TIFR)
UAS-GKVK Campus
Bellary Road
Bangalore 560065
INDIA
Kannan, Sankar; Birla Institute of Technology and Science
Pilani
Nemoto, Wataru; Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology, 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan
Fukui, Kazuhiko; Computational Biology Research Center
Advanced Industrial Science and Technology
2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan
Sowdhamini, Ramanathan; National Centre for Biological Sciences (TIFR)
UAS-GKVK Campus
Bellary Road
Bangalore 560065
INDIA
olfaction; genome-wide survey; three-dimensional modelling; membrane proteins
<p class="PreformattedText">Olfactory Receptors (ORs) are members of the Class A rhodopsin like G-protein coupled receptors (GPCRs) which are the initial players in the signal transduction cascade, leading to the generation of nerve impulses transmitted to the brain and resulting in the detection of odorant molecules. Despite the accumulation of thousands of olfactory receptor sequences, no crystal structures of ORs are known tο date. However, the recent availability of crystallographic models of a few GPCRs allows us to generate homology models of ORs and analyze their amino acid patterns, as there is a huge diversity in OR sequences. In this study, we have generated three-dimensional models of 100 representative ORs from <em>Homo sapiens</em>, <em>Mus musculus</em>, <em>Drosophila melanogaster</em>, <em>Caenorhabditis elegans</em> and <em>Sacharomyces cerevisiae</em> which were selected on the basis of a composite classification scheme and phylogenetic analysis. The crystal structure of bovine rhodopsin was used as a template and it was found that the full-length models have more than 90% of their residues in allowed regions of the Ramachandran plot. The structures were further used for analysis of conserved residues in the transmembrane and extracellular loop regions in order to identify functionally important residues. Several ORs are known to be functional as dimers and hence dimer interfaces were predicted for OR models to analyse their oligomeric functional state.</p>
Lorem Ipsum Press
DBT-AIST
2012-10-21 03:53:39
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/57
Journal of Molecular Biochemistry; Vol 1, No 3
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/57/153
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/57/286
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/60
2012-10-21T03:53:39Z
JmolBiochem:REV
"121018 2012 eng "
dc
Role of Cbl-associated protein/ponsin in receptor tyrosine kinase signaling and cell adhesion
Tomasovic, Ana; Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany
Kurrle, Nina; Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany
Banning, Antje; Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany
Tikkanen, Ritva; Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany
Receptor tyrosine kinase; insulin; diabetes; cell adhesion
<span style="font-family: Times New Roman; font-size: small;"> </span><p>The Cbl-associated protein/ponsin (CAP) is an adaptor protein that contains a so-called Sorbin homology (SoHo) domain and three Src homology 3 (SH3) domains which are engaged in diverse protein-protein interactions. CAP has been shown to function in the regulation of the actin cytoskeleton and cell adhesion and to be involved in the differentiation of muscle cells and adipocytes. In addition, it participates in signaling pathways through several receptor tyrosine kinases such as insulin and neurotrophin receptors. In the last couple of years, several studies have shed light on the details of these processes and identified novel interaction partners of CAP. In this review, we summarize these recent findings and provide an overview on the function of CAP especially in cell adhesion and membrane receptor signaling.</p><span style="font-family: Times New Roman; font-size: small;"> </span>
Lorem Ipsum Press
Deutsche Forschungsgemeinschaft DFG, von Behring Röntgen Foundation, State of Hessen
2012-10-21 03:53:39
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/60
Journal of Molecular Biochemistry; Vol 1, No 3
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/65
2012-10-21T03:53:39Z
JmolBiochem:COMM
"121018 2012 eng "
dc
Molecular mechanism-based model to enhance outcomes of dietary intervention studies for disease prevention
Dey, Moul; Molecular Nutrition and Nutrigenomics research program, Department of Health & Nutritional Sciences, South Dakota State University, Box 2203, Brookings, SD 57007, USA
molecular nutrition; nutritional biochemisty; nutrigenomics
<span style="font-family: Times New Roman; font-size: small;"> </span><p>Advances in “omics”-based fields have produced an explosion of new information, fueling high expectations for improved public and individualized health. Unfortunately, there exists a widening gap between basic biochemistry and “omics”-based population research, with both disciplines failing to translate their full potential impact to human health applications. A paucity of comprehensive study systems is one of the many roadblocks faced by translational research today. This commentary will highlight the current status of such research, particularly emphasizing the role of nutrigenomics.</p><span style="font-family: Times New Roman; font-size: small;"> </span>
Lorem Ipsum Press
NIH, AES-USDA
2012-10-21 03:53:39
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/65
Journal of Molecular Biochemistry; Vol 1, No 3
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/69
2012-10-21T03:53:39Z
JmolBiochem:ART
"121018 2012 eng "
dc
Identification of drought-induced transcription factors in peanut (Arachis hypogaea L.)
Dang, Phat M; USDA-ARS, National Peanut Research Laboratory
Chen, Charles Y; Department of Agronomy and Soils, Auburn University
Holbrook, Corley C; USDA, ARS,Crop Genetics and Breeding Research Unit, Tifton, GA
peanut; drought; gene-expression; PCR
<p>Transcription factors play key roles in the regulation of genes involved in normal development as well as tolerance to biotic and abiotic stresses. Specific transcription factors that are induced in peanut under drought conditions have not been identified. The objectives of this study were to compare gene-expression patterns of various transcription factors of a drought tolerant versus a susceptible peanut genotype under drought conditions and to identify transcripts that were regulated in a drought dependent manner. Twelve putative transcription factors were identified and real-time PCR analysis was performed which resulted in the identification of three unique transcripts in which ahERF1 was highly induced in the recovery stage; ahERF7 and ahERF8 were also highly induced by drought and returned to nominal levels after recovery. These sequences contain DNA binding domains that are present in the APETALA2/Ethelene Responsive Factors (AP2/ERF) family of transcription factors which have been shown to be induced by stress. Induction levels and patterns of gene-expression of ahERF1, ahERF7 and ahERF8 may be used to select plants that may have higher drought tolerance.</p>
Lorem Ipsum Press
USDA, ARS
2012-10-21 03:53:39
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/69
Journal of Molecular Biochemistry; Vol 1, No 3
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/69/291
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/75
2012-10-21T05:25:28Z
JmolBiochem:ART
"121018 2012 eng "
dc
Spectral analysis and crystal structure of two substituted spiro acenaphthene structures
Suresh, J.
Vishnu Priya, R.; Department of Physics, The Madura College, Madurai 625 011, India
Sivakumar, S.; Department of Organic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625 021, India
Ranjith Kumar, R.; Department of Organic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625 021, India
Crystal structure; conformation; coordination interactions; hydrogen bond
<p>The crystal structure of spiro[2.2"]acenaphthene-1"-onespiro[3.3']-5'-(2-bromophenylmethylidene)-1'-methylpiperidin-4'-one-4-(2-bromophenyl)octahydroindolizine <strong>(Ia)</strong> and spiro[2.2"]acenaphthene-1"-onespiro[3.3']-5'-(4-bromophenylmethylidene)-1'-methylpiperidin-4'-one-4-(4-bromophenyl)octahydroindolizine <strong>(Ib)</strong> were elucidated by single crystal X ray diffraction. Compound <strong>(Ia)</strong>, C<sub>37</sub>H<sub>32</sub>Br<sub>2</sub>N<sub>2</sub>O<sub>2</sub>, crystallizes in the Orthorombic system, space group P2<sub>1</sub>2<sub>1</sub>2<sub>1 </sub>with a = 9.6989(5) Å, b = 16.3615(9) Å, c = 19.8997(11) Å and Z = 4. Compound <strong>(Ib)</strong>, having the same molecular formula, crystallizes in the monoclinic system, space group P 2<sub>1</sub>/n with a = 8.3169(5) Å, b = 16.9397(9) Å, c= 22.2466(13) Å, β = 96.943(3)° and Z = 4. The central piperidine ring adopts twisted conformation, the piperidine of octahydroindolizine ring is in half chair conformation and the pyrrole ring is in twisted envelope conformation in both the compounds (Ia) and (Ib). Details of the preparation, NMR and crystal structure determination and intra- and inter- molecular interactions of the compounds are given.</p>
Lorem Ipsum Press
2012-10-21 03:53:39
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/75
Journal of Molecular Biochemistry; Vol 1, No 3
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/75/217
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/75/218
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/94
2013-02-22T18:18:49Z
JmolBiochem:ED
"130220 2013 eng "
dc
Limitations of the peer review system and possible alternatives
Kyriazis, Marios; British Longevity Society, Dunstable, United Kingdom
peer review, open, post-publication
Lorem Ipsum Press
2013-02-22 18:18:49
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/94
Journal of Molecular Biochemistry; Vol 2, No 1 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/88
2013-02-22T18:18:49Z
JmolBiochem:LETT
"130220 2013 eng "
dc
Zooming (a little) out of the M-theory
Piergentili, Roberto; Istituto di Biologia e Patologia Molecolari del CNR; Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma; Rome, Italy.
reductionism; holism; Systems Biology
Lorem Ipsum Press
2013-02-22 18:18:49
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/88
Journal of Molecular Biochemistry; Vol 2, No 1 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/52
2020-02-17T19:05:03Z
JmolBiochem:ART
"130220 2013 eng "
dc
Alpha-linolenic acid regulates the growth of breast and cervical cancer cell lines through regulation of NO release and induction of lipid peroxidation
Deshpande, Rashmi; IRSHA
Mansara, Prakash; IRSHA
Suryavanshi, Snehal; IRSHA, Bharati Vidyapeeth University
Kaul-Ghanekar, Ruchika; IRSHA, Bharati Vidyapeeth University
Alpha Linolenic acid; ALA; Nitric Oxide; NO; Lipid peroxidation; LPO
<p>In the present work, we have analyzed the effect of the essential fatty acid, alpha linolenic acid (ALA) on nitric oxide release as well as induction of lipid peroxidation in breast (MCF-7 and MDA-MB-231) and cervical (SiHa and HeLa) cancer cell lines. ALA-treated cells showed a dose-dependent decrease in cell viability in both breast and cervical cancer cell lines without affecting the viability of non-cancerous transformed HEK 293 cells. Both types of cancer cells treated with ALA demonstrated a significant reduction in nitric oxide (NO) release with a simultaneous increase in lipid peroxidation (LPO). This was followed by a decrease in the mitochondrial membrane potential as well as activation of caspase 3 leading to apoptosis. Thus, ALA regulated the growth of cancer cell lines through induction of lipid peroxidation and modulation of nitric oxide release resulting in apoptosis.</p>
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IRSHA, Bharati Vidyapeeth University
2013-02-22 18:18:49
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/52
Journal of Molecular Biochemistry; Vol 2, No 1 (2013)
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/52/332
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/59
2013-02-22T18:29:15Z
JmolBiochem:ART
"130220 2013 eng "
dc
STAT3 inhibition induces apoptosis in cancer cells independent of STAT1 or STAT2
Shodeinde, Adetola; University of Medicine and Dentistry of New Jersey
Ginjupalli, Kalyani
Lewis, H. Dan
Riaz, Sheraz
Barton, Beverly E; University of Medicine and Dentistry of New Jersey
STAT1; STAT2; STAT3; prostate cancer; pancreatic cancer; inhibitors
<p>Signal transducers and activators of transcription (STATs) were originally discovered as mediators of signal transduction. Persistent aberrant activation of STAT3 is part of the malignant phenotype of hormone-refractory prostate cancer and pancreatic cancer; this is thought to be mediated by homodimers of phosphorylated STAT3, which translocate to the nucleus. One consequence of persistently-activated STAT3 in malignant cells is that they depend upon it for survival. STAT3 is observed to heterodimerize with STAT1 and STAT2; however the contributions of STAT3:STAT1 and STAT3:STAT2 heterodimers to the survival of malignant cells have not been investigated in detail.</p> <p>Previously we reported that single-stranded oligonucleotides containing consensus STAT3 binding sequences (13410 and 13411) were more effective for inducing apoptosis in prostate cancer cells than antisense STAT3 oligonucleotides. Control oligonucleotides (scrambled sequences) had no effect. STAT3-inhibiting oligonucleotide 13410, but not scrambled-sequence oligonucleotides, induced apoptosis in pancreatic cancer cells as well. Here we report that 13410 and derivative olignucleotides induced apoptosis in STAT1-null and STAT2-null fibrosarcoma cell lines U3A and U6A, as well as in the parental fibrosarcoma cell line 2fTGH. The cell lines expressed constitutively-activated STAT3 and depended on its activity for survival. Forty-eight hr after transfection of 13410 or related oligonucleotides, significant apoptosis was observed in 2fTGH, U3A and U6A cells. Scrambled-sequence oligonucleotides had no effect on survival. These data indicate that neither STAT1 nor STAT2 play significant roles in the maintenance of these cells, and by extension that STAT3:STAT1 and STAT3:STAT2 heterodimers regulate a different set of genes from STAT3:STAT3 homodimers.</p> <p> </p><p> </p>
Lorem Ipsum Press
NIH, Elsa U. Pardee Foundation, Department of Veterans Affairs (BEB)
2013-02-22 18:18:49
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/59
Journal of Molecular Biochemistry; Vol 2, No 1 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/81
2013-02-22T18:18:49Z
JmolBiochem:ART
"130220 2013 eng "
dc
How complex an intron may be? The example of the first intron of the CTP synthase gene of Drosophila melanogaster
Piergentili, Roberto; Sapienza - Università di Roma
small interfering RNA; antisense RNA; nucleotide pool; CG6854; embryogenesis; neurogenesis
<p>In eukaryotes, maturation of primary transcripts into mature messenger RNAs involves the elimination of parts of the gene called ‘introns’. The biological significance of introns is not yet completely understood. It has been demonstrated that introns may contain other genes, or regulatory sequences that may be involved in transcriptional control, or also being involved in alternative splicing mechanisms. However, these functions explain the role of only a small number of them, and it is very difficult to formulate any generalization. The CTP synthase gene of Drosophila melanogaster is characterized by the presence of a long first intron (approximately 7.2 kilobases) whose role is currently unknown. In the present report we analyzed in silico the content of this intron, and found that it contains at least three interesting sub-sequences. Two of them are homologous to the CTP synthase itself and to a putative nucleotide pyrophosphatase, respectively. The third is a short stretch of DNA able to fold into a thermodynamically stable hairpin and showing homology with other 19 sequences from 21 genes inside the D. melanogaster genome. These findings suggest a complex yet very accurate way of controlling gene expression inside the fruit fly.</p>
Lorem Ipsum Press
2013-02-22 18:18:49
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/81
Journal of Molecular Biochemistry; Vol 2, No 1 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/84
2013-02-22T18:18:49Z
JmolBiochem:ART
"130220 2013 eng "
dc
Motifs within the CA-repeat-rich region of Surfactant Protein B (SFTPB) intron 4 differentially affect mRNA splicing
Yang, Wenjun; Center for Host Defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, Penn State Hershey College of Medicine, Pennsylvania State University, Hershey, Pennsylvania.
Ni, Lan; Center for Host Defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, Penn State Hershey College of Medicine, Pennsylvania State University, Hershey, Pennsylvania.
Silveyra, Patricia; Center for Host Defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, Penn State Hershey College of Medicine, Pennsylvania State University, Hershey, Pennsylvania.
Wang, Guirong; Center for Host Defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, Penn State Hershey College of Medicine, Pennsylvania State University, Hershey, Pennsylvania.
Noutsios, Georgios T.; Center for Host Defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, Penn State Hershey College of Medicine, Pennsylvania State University, Hershey, Pennsylvania.
Singh, Anamika; Center for Host Defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, Penn State Hershey College of Medicine, Pennsylvania State University, Hershey, Pennsylvania.
DiAngelo, Susan L.; Center for Host Defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, Penn State Hershey College of Medicine, Pennsylvania State University, Hershey, Pennsylvania.
Sanusi, Olabisi; Center for Host Defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, Penn State Hershey College of Medicine, Pennsylvania State University, Hershey, Pennsylvania.
Raval, Manmeet; Center for Host Defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, Penn State Hershey College of Medicine, Pennsylvania State University, Hershey, Pennsylvania.
Floros, Joanna; Center for Host Defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics; Department of Obstetrics and Gynecology, Penn State Hershey College of Medicine, Pennsylvania State University, Hershey, Pennsylvania.
RNA; SP-B; secondary structure; SRp20; SRSF3; mRNA splicing
<p>The first half of the surfactant protein B (SP-B) gene intron 4 is a CA-repeat-rich region that contains 11 motifs. To study the role of this region on SP-B mRNA splicing, minigenes were generated by systematic removal of motifs from either the 5’ or 3’ end. These were transfected in CHO cells to study their splicing efficiency. The latter was determined as the ratio of completely to incompletely spliced SP-B RNA. Our results indicate that SP-B intron 4 motifs differentially affect splicing. Motifs 8 and 9 significantly enhanced and reduced splicing of intron 4, respectively. RNA mobility shift assays performed with a Motif 8 sequence that contains a CAUC cis-element and cell extracts resulted in a RNA:protein shift that was lost upon mutation of the element. Furthermore, <em>in silico</em> analysis of mRNA secondary structure stability for minigenes with and without motif 8 indicated a correlation between mRNA stability and splicing ratio. We conclude that differential loss of specific intron 4 motifs results in one or more of the following: a) altered splicing, b) differences in RNA stability and c)changes in secondary structure. These, in turn, may affect SP-B content in lung health or disease.</p>
Lorem Ipsum Press
NIH HL-34788 grant
2013-02-22 18:18:49
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/84
Journal of Molecular Biochemistry; Vol 2, No 1 (2013)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/84/338
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/84/339
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/84/340
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/87
2013-02-22T18:18:49Z
JmolBiochem:REV
"130220 2013 eng "
dc
Tumor markers in finding recurrent disease in colorectal cancer: a diagnostic review
Verberne, Charlotte; University Medical Center Groningen
Jong, Helma de; University Medical Center Groningen
Grossmann, Irene; University Medical Center Groningen
Bock, Geertruida de; University Medical Center Groningen
Wiggers, Theo; University Medical Center Groningen
Kema, Ido; University Medical Center Groningen
Muller Kobold, Anneke; University Medical Center Groningen
tumor marker; CEA; clinical biochemistry; colorectal cancer
<p>Aim: In the search for evidence-based follow-up of patients after resection for colorectal cancer, numerous tumor markers have been proposed. This review has evaluated these markers and comments on the diagnostic accuracy in finding recurrent disease in relation to Carcino-Embryonic Antigen (CEA).</p> <p>Methods: A comprehensive literature review (1985-2010) was performed by two independent reviewers. Sensitivity and specificity of markers mentioned in the articles were checked by recalculation. A validated quality score system was used to estimate study quality.</p> <p>Results: Seventeen studies focusing on eight different markers were included. Three markers were shown to have comparable or better accuracy than CEA: TPA, CA 242 and CA 72-4 in at least one study. These three markers, from four independent studies, showed a tumor marker sensitivity of > 60% in combination with an outperformance of CEA in follow-up. These results were not confirmed by six other studies investigating the same markers.</p> <p>Conclusion: This review revealed three tumor markers other than CEA that have been shown to adequately indicate recurrences in colorectal cancer. However, comparability of studies was difficult. Therefore a prospective study of these markers seems necessary to investigate their real value, and to overcome design and inclusion biases.</p>
Lorem Ipsum Press
2013-02-22 18:18:49
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/87
Journal of Molecular Biochemistry; Vol 2, No 1 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/89
2013-02-22T18:18:49Z
JmolBiochem:REV
"130220 2013 eng "
dc
Metabolism of triacylglycerols in Rhodococcus species: insights from physiology and molecular genetics
Alvarez, Héctor M.; University of Patagonia
Silva, Roxana A.
Herrero, Marisa
Hernández, Martín A.
Villalba, María S.
Rhodococcus, triacylglycerols, biosynthesis and degradation genes
<p>Rhodococcus bacteria possess the ability to accumulate variable amounts of triacylglycerols (TAG) during growth on diverse carbon sources. The evolution seems to have selected these microorganisms as specialists in the accumulation of TAG among bacteria, since their biochemistry is efficiently designed for the biosynthesis and mobilization of these lipids. Detailed research of rhodococcal TAG metabolism started only a few years ago; thus, the fundamental understanding of this process and its regulation remains to be clarified. However, some interesting advances in the basic knowledge on TAG metabolism in rhodococci have been made. Most studies have focused on the physiology of TAG biosynthesis and mobilization in rhodococci. Only recently, some advances in molecular biology and genetics on TAG metabolism occurred as a result of the increasing available genomic information and the development of new genetic tools for rhodococci. These studies have been focused principally on some enzymes of TAG biosynthesis, such as the wax esters/diacylglycerolacyltransferases (WS/DGAT) and TAG granule-associated proteins. In this context, the most relevant achievements of basic research in the field have been summarized in this review article.</p>
Lorem Ipsum Press
2013-02-22 18:18:49
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/89
Journal of Molecular Biochemistry; Vol 2, No 1 (2013)
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Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/106
2013-06-23T06:49:29Z
JmolBiochem:ED
"130622 2013 eng "
dc
An update on virology and emerging viral epidemics
Vlachakis, Dimitrios
Karozou, Argiro
Kossida, Sophia
Lorem Ipsum Press
2013-06-23 06:49:30
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/106
Journal of Molecular Biochemistry; Vol 2, No 2 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/90
2013-06-23T06:49:30Z
JmolBiochem:ART
"130622 2013 eng "
dc
Genome sequences of triacylglycerol metabolism in Rhodococcus as a platform for comparative genomics
Villalba, María S
Hernández, Martín A
Silva, Roxana A
Alvarez, Héctor M.; University of Patagonia
Rhodococcus, triacylglycerols, comparative genomics, biosynthesis and degradation genes
<p>Bacteria belonging to the Rhodococcus genus are usually able to synthesize and accumulate variable amounts of triacylglycerols (TAG) from diverse carbon sources. Although some significant advances in the basic knowledge on TAG metabolism in rhodococci have been made, the fundamental understanding of this process and its regulation remains to be clarified. The abundantly available genomic information for several rhodococcal species provides the possibility for comparative genome analysis on the occurrence and distribution of key genes and pathways involved in TAG metabolism. Our bioinformatic analyses of available databases from six rhodococcal strains demonstrated that genes/enzymes for reactions related to TAG biosynthesis and degradation, and fatty acid β-oxidation are surprisingly abundant in rhodococcal genomes. Several genes/enzymes of glycerolipids and fatty acid metabolism are highly represented in the analyzed genomes. A number of previously undescribed, new putative genes for glycerolipid metabolism in rhodococci have been identified and the size of each family has been estimated.</p>
Lorem Ipsum Press
2013-06-23 06:49:30
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/90
Journal of Molecular Biochemistry; Vol 2, No 2 (2013)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/90/314
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/90/315
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/103
2013-06-23T06:49:30Z
JmolBiochem:REV
"130622 2013 eng "
dc
Somatostatin and Epidermal Growth Factor Receptors: Implications in Breast Cancer
Kumar, Ujendra; Faculty of Pharmaceutical sciences, University of British Columbia
Kharmate, Geetanjali; Faculty of Pharmaceutical Sciences
Post Doctoral fellow
Breast Cancer, Dimerization, Heterodimerization, Epidermal growth factor, Epidermal growth factor Receptors, Somatostatin, Somatostatin Receptors
<p>Despite several advances, the underlying mechanism of complexity of breast cancer progression still remains elusive. In addition to the genetic predisposition, several growth factor receptors including insulin growth factor receptor (IGF), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) relaying proliferative signals are accountable for disease progression. Epidermal growth factor receptors (EGFRs, or commonly known as ErbBs), members of the receptor tyrosine kinase family (RTKs), play a central role in tumor growth, progression and metastatic disease. Typically, agonist dependent activation of EGFR results in receptor phosphorylation, homo- and/or heterodimerization and modulation of signaling pathways leading to cell proliferation, survival and metastasis. Targeting one or multiple steps in EGFR-mediated tumor progression may serve as a better approach in drug therapies. Unlike EGFRs, G-protein coupled somatostatin receptors (SSTRs) have been recognized as negative regulators of breast tumors. The activation of SSTRs modulates downstream signaling responsible for tumor growth and consequent cytostatic or cytotoxic effects on tumor proliferation. SSTR subtypes are well characterized to form homo-and/or heterodimers within the same family as well as with other GPCRs. Clinically, the chimeric molecule targeting both SSTR5 and dopamine receptors (specifically dopamine receptor 2) is in use for the treatment of pituitary tumors. This review describes the interplay between SSTRs and EGFR and the potential role of such cross talk in attenuation of EGFR-mediated signaling pathways involved in tumorigenesis. Furthermore, recent findings supporting the role of SSTR in EGFR-mediated signaling in tumor biology are discussed in detail.</p>
Lorem Ipsum Press
2013-06-23 06:49:30
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/103
Journal of Molecular Biochemistry; Vol 2, No 2 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/95
2013-06-23T06:49:30Z
JmolBiochem:ART
"130623 2013 eng "
dc
Molecular modelling study of the 3D structure of the biglycan core protein, using homology modelling techniques
Vlachakis, Dimitrios
Champeris Tsaniras, Spyridon
Feidakis, Christos
Kossida, Sophia
Biglycan; dermatan sulphate; homology modelling; molecular dynamics simulation; small leucine reach proteins (SLRPs)
<p>Herein we report the establishment of the 3D structure of the biglycan core protein, using conventional homology molecular modelling techniques. The 3D model has been structurally optimised via molecular dynamics. It was found that the final model of biglycan resembles in structure its template protein bearing a set of distinct parallel β-sheet structure patterns. The biglycan model bears a very hydrophobic amino acid region towards its inner cavity that acquires an arc-like structure. The external domain of the biglycan model is made up of hydrophilic residues that are exposed to the water solvent. It is those hydrophilic residues that are responsible for their interaction with polysaccharide polymers. Overall comparison of the model of biglycan to the recently determined x-ray structure of the same protein returns a very low Root Mean Square Deviation (RMSD), which confirms the viability of the model and its reliability as a platform for the study biglycan interactions.</p><p> </p>
Lorem Ipsum Press
2013-06-23 06:49:30
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/95
Journal of Molecular Biochemistry; Vol 2, No 2 (2013)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/95/344
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/95/345
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/95/346
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/95/347
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/95/348
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/95/349
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/95/350
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/104
2013-10-17T16:42:28Z
JmolBiochem:COMM
"131017 2013 eng "
dc
Innovation, nanotechnology and industrial sustainability by the use of natural underutilized byproducts
Morganti, Pierfrancesco; Professor of Skin Pharmacology
Dermatology Institute
University of Napoli, Italy
nanoscience, nanotechnology, chitin nanofibrils, lignin
<p class="Body10">Nanotechnology can have a positive impact on environmental problems and aid in the development of new green technologies. In this direction, two EU projects have been set up, called BioMimetic and n-Chitopack as well as an Italian one, named Chitofarma. Chitin nanofibrils (CN), coming from fishery waste, and biopolymers, extracted from vegetable biomass, are among the basic polymers used. These raw materials of natural origin are skin and environmentally-friendly. They can be conjugated or cross-linked by bio-mimetic processes to create innovative products, such as specialized cosmetics, food packaging and non-woven antiaging tissues. A description of these research projects together with their expected results will be briefly reported.</p> <p> </p><p class="Body10"> </p><p class="Body1"> </p>
Lorem Ipsum Press
2013-10-17 00:00:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/104
Journal of Molecular Biochemistry; Vol 2, No 3 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/97
2013-12-06T05:44:31Z
JmolBiochem:REV
"131017 2013 eng "
dc
RETRACTED ARTICLE: The biochemistry of male homosexuality
Kotsi, Ioanna
Vlachaki, Chrisanthy
Tsitsas, George Dimosthenis; Harokopio University of Athens
male homosexuality, fraternal birth order, maternal immune hypothesis, heterosexuality, homosexuality
<p>Homosexuality is a common occurrence in humans and other species. It is a complex, universal phenomenon whose genetic and evolutionary basis is poorly understood. Despite great progress in the sciences, our understanding of the determinants of sexual orientation and, more specifically, of male homosexuality, is incomplete. In this paper, we have reviewed the biological causes of male homosexuality, which may provide clues for further research in this field, as provable biological or genetic differences between heterosexuals and homosexuals that weren't caused by their behaviour haven’t been found.</p><p><span><strong>This article has been retracted due to plagiarism. An erratum will be published in Volume 3, Issue 1 (2014).</strong></span></p>
Lorem Ipsum Press
2013-10-17 00:00:00
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/97
Journal of Molecular Biochemistry; Vol 2, No 3 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/107
2013-10-18T13:53:07Z
JmolBiochem:ART
"131017 2013 eng "
dc
Insights into the structure and 3D spatial arrangement of the b-ketoacyl carrier protein synthases
Vlachakis, Dimitrios
Champeris Tsaniras, Spyridon
Kossida, Sophia
b-ketoacyl carrier protein synthases; anti-Plasmodium drug targets; molecular modelling; bioinformatics
<p>The b-ketoacyl carrier protein synthases (the KAS enzymes) are key enzymes that can be used as potential anti-Plasmodium drug targets. In bacteria, three KAS enzymes have been identified (KAS I, KAS II and KAS III), whilst in Plasmodium a KAS I/II and KAS III enzyme has been reported. The protein has a total of four active sites, which have been found to be different to each other, rather than four copies of the same active site. The active sites differ not only in the type of interaction they establish with the ligand, but, in the case of Cerulenin as a ligand, the active sites of the KAS I/II enzyme also differ in the number of residues involved in the ligand protein interaction. This is very interesting biochemically, because these differences imply that the affinity of each active site for binding to the ligand might be different as well.</p> <p> </p><p> </p>
Lorem Ipsum Press
2013-10-17 00:00:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/107
Journal of Molecular Biochemistry; Vol 2, No 3 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/105
2013-10-17T16:42:28Z
JmolBiochem:ART
"131017 2013 eng "
dc
Spatial patterns of phosphorylation-dependent TDP-43-immunoreactive neuronal cytoplasmic inclusions (NCI) in frontotemporal lobar degeneration with TDP-43 proteinopathy (FTLD-TDP)
Armstrong, Richard; Aston University
Frontotemporal lobar degeneration; TDP-43 proteinopathy; FTLD-TDP; Transactive response TAR DNA-binding protein of 43 kDa; TDP-43; Neuronal cytoplasmic inclusions; NCI
<p>The transactive response (TAR) DNA-binding protein of 43kDa (TDP-43) is an RNA binding protein encoded by the TARDPB gene. Abnormal aggregations of TDP-43 in neurons in the form of neuronal cytoplasmic inclusions (NCI) are the pathological hallmark of frontotemporal lobar degeneration with TDP-43 proteinopathy (FTLD-TDP). To investigate the role of TDP-43 in FTLD-TDP, the spatial patterns of the NCI were studied in frontal and temporal cortex of FTLD-TDP cases using a phosphorylation dependent anti-TDP-43 antibody (pTDP-43). In many regions, the NCI formed clusters and the clusters were distributed regularly parallel to the tissue boundary. In about 35% of cortical regions, cluster size of the NCI was within the size range of the modular columns of the cortex. The spatial patterns of the pTDP-immunoreactive inclusions were similar to those revealed by a phosphorylation-independent anti-TDP-43 antibody and also similar to inclusions characterized by other molecular pathologies such as tau, a-synuclein and ‘fused in sarcoma’ (FUS). In conclusion, the data suggest degeneration of cortical and hippocampal anatomical pathways associated with accumulation of cellular pTDP-43 is characteristic of FTLD-TDP. In addition, the data are consistent with the hypothesis of cell to cell transfer of pTDP-43 within the brain.</p> <p> </p><p> </p>
Lorem Ipsum Press
Dr N Cairns, Washington University, St Louis; Technical staff, Depts Neurology, pathology, Immunology, Washington University, St Louis
2013-10-17 00:00:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/105
Journal of Molecular Biochemistry; Vol 2, No 3 (2013)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/110
2014-03-03T12:18:18Z
JmolBiochem:ART
"140228 2014 eng "
dc
The organization of metabolic genotype space facilitates adaptive evolution in nitrogen metabolism
Wagner, Andreas; University of Zurich
Andriasyan, Vardan
Barve, Aditya
metabolism; evolution; reaction networks; flux balance analysis; innovation
<p>A metabolism is a complex chemical reaction system, whose metabolic genotype – the DNA encoding the enzymes catalyzing these reactions – can be compactly represented by its complement of metabolic reactions. Here, we analyze a space of such metabolic genotypes. Specifically, we study nitrogen metabolism and focus on nitrogen utilization phenotypes that are defined through the viability of a metabolism – its ability to synthesize all essential small biomass precursors – on a given combination of sole nitrogen sources. We randomly sample metabolisms with known phenotypes from metabolic genotype space with the aid of a method based on Markov Chain Monte Carlo sampling. We find that metabolisms viable on a given nitrogen source or a combination of nitrogen sources can differ in as much as 80 percent of their reactions, but can form networks of genotypes that are connected to one another through sequences of single reaction changes. The reactions that cannot vary in any one metabolism differ among metabolisms, and include a small core of “absolutely superessential” reactions that are required in all metabolisms we study. Only a small number of reaction changes are needed to reach the genotype network of one metabolic phenotype from the genotype network of another metabolic phenotype. Our observations indicate deep similarities between the genotype spaces of macromolecules, regulatory circuits, and metabolism that can facilitate the origin of novel phenotypes in evolution.</p> <p> </p><p> </p>
Lorem Ipsum Press
Swiss National Science Foundation
2014-03-03 00:00:00
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/110
Journal of Molecular Biochemistry; Vol 3, No 1 (2014)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/110/421
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/110/422
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/110/423
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/111
2014-03-03T12:18:18Z
JmolBiochem:ART
"140228 2014 eng "
dc
The Niemann-Pick C1 and caveolin-1 proteins interact to modulate efflux of low density lipoprotein-derived cholesterol from late endocytic compartments
Jelinek, David
Heidenreich, Randy A
Orlando, Robert A
Garver, William S; University of New Mexico
caveolin-1; cholesterol; endosomes; Niemann-Pick C1
<p>The Niemann-Pick C1 (NPC1) protein has a central role in regulating the efflux of lipoprotein-derived cholesterol from late endosomes/lysosomes and transport to other cellular compartments. Since the NPC1 protein has been shown to regulate the transport of cholesterol to cellular compartments enriched with the ubiquitous cholesterol-binding and transport protein caveolin-1, the present study was performed to determine whether the NPC1 and caveolin-1 proteins interact and function to modulate efflux of low density lipoprotein (LDL)-derived cholesterol from endocytic compartments. To perform these studies, normal human fibroblasts were grown in media with lipoprotein-deficient serum (LPDS) or media with LPDS supplemented with purified human LDL. The results indicated reciprocal co-immunoprecipitation and partial co-localization of the NPC1 and caveolin-1 proteins that was decreased when fibroblasts were grown in media with LDL. Consistent with interaction of the NPC1 and caveolin-1 proteins, a highly conserved caveolin-binding motif was identified within a cytoplasmic loop located adjacent to the sterol-sensing domain (SSD) of the NPC1 protein. To examine the functional relevance of this interaction, fibroblasts were transfected with caveolin-1 siRNA and found to accumulate increased amounts of LDL-derived cholesterol within late endosomes/lysosomes. Together, this report presents novel results demonstrating that the NPC1 and caveolin-1 proteins interact to modulate efflux of LDL-derived cholesterol from late endocytic compartments.</p>
Lorem Ipsum Press
National Institutes of Health (R21 DK071544); Ara Parseghian Medical Research Foundation;
2014-03-03 00:00:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/111
Journal of Molecular Biochemistry; Vol 3, No 1 (2014)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/112
2014-03-03T12:18:18Z
JmolBiochem:ART
"140228 2014 eng "
dc
3D structural analysis of proteins using electrostatic surfaces based on image segmentation
Vlachakis, Dimitrios
Champeris Tsaniras, Spyridon
Tsiliki, Georgia
Megalooikonomou, Vasileios
Kossida, Sophia
protein function; molecular docking; three-dimensional structure; image segmentation; molecular surface; similarity searches
<p>Herein, we present a novel strategy to analyse and characterize proteins using protein molecular electrostatic surfaces. Our approach starts by calculating a series of distinct molecular surfaces for each protein that are subsequently flattened out, thus reducing 3D information noise. RGB images are appropriately scaled by means of standard image processing techniques whilst retaining the weight information of each protein’s molecular electrostatic surface. Then homogeneous areas in the protein surface are estimated based on unsupervised clustering of the 3D images, while performing similarity searches. This is a computationally fast approach, which efficiently highlights interesting structural areas among a group of proteins. Multiple protein electrostatic surfaces can be combined together and in conjunction with their processed images, they can provide the starting material for protein structural similarity and molecular docking experiments.</p> <p> </p><p> </p>
Lorem Ipsum Press
2014-03-03 00:00:00
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/112
Journal of Molecular Biochemistry; Vol 3, No 1 (2014)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/113
2014-03-03T12:18:18Z
JmolBiochem:ART
"140228 2014 eng "
dc
The grape fruit flavonone naringin protects mice against doxorubicin-induced cardiotoxicity
Jagetia, Ganesh Chandra; Mizoram University
Reddy, Tiyagura Koti
Doxorubicin; mice; cardiotoxicity; glutathione; PARP; DNA adducts
<p>Doxorubicin (DOX), an anthracycline drug widely used for the treatment of various cancers, causes a cumulative dose-dependent cardiac toxicity that is characterized by an irreversible cardiomyopathy and congestive heart failure. The cardioprotective effect of 2.5, 5, 7.5 and 10 mg/kg naringin (NIN) was studied in mice treated with 15 mg/kg DOX. The animals were killed 30 h after DOX treatment. The latter induced acute cardiotoxicity indicated by a significant elevation in glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), creatine kinase (CK-MB) and lactate dehydrogenase (LDH) in mice serum. Treatment of mice with NIN before DOX administration significantly reduced serum levels of GPT, GOT, CK-MB and LDH indicating that NIN protected against the DOX-induced cardiotoxicity. DOX induced a significant increase in the 8-OHdG DNA adducts and the PARP activity in the heart and liver of mice, whereas NIN treatment of mice before DOX administration significantly reduced 8-OHdG DNA adducts and PARP activity in the heart and liver. Similarly, NIN inhibited the DOX-induced decline in the glutathione concentration, catalase and superoxide dismutase activities and abated DOX-induced lipid peroxidation in the heart and liver of mice. Intraperitoneal administration of 1.25 mg/kg DOX significantly elevated survival of Ehrlich ascites carcinoma (EAC) bearing mice, whereas the combination of 10 mg/kg NIN with DOX did not alter the tumor cell growth, median survival time or average survival time of tumor bearing mice when compared to DOX treatment alone, indicating that NIN does not interfere with the antineoplastic activity of DOX. Our study demonstrates that naringin reduced the doxorubicin-induced cardiotoxicity, without affecting its antineoplastic activity, which may be due to reduction in the DOX-induced 8-OHdG DNA adducts and PARP activity, increase in the antioxidant enzymes and alleviation of lipid peroxidation by naringin.</p>
Lorem Ipsum Press
2014-03-03 00:00:00
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/113
Journal of Molecular Biochemistry; Vol 3, No 1 (2014)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/115
2014-03-03T12:18:18Z
JmolBiochem:CORR
"140228 2014 eng "
dc
Erratum
[Erratum], [Erratum]
Lorem Ipsum Press
2014-03-03 00:00:00
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/115
Journal of Molecular Biochemistry; Vol 3, No 1 (2014)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/117
2014-06-30T12:38:35Z
JmolBiochem:COMM
"140630 2014 eng "
dc
On mechanical force generated by EF-G-catalyzed ribosome translocation
Xie, Ping; Key Laboratory of Soft Matter Physics and Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China
riboosome; mechanical force; EF-G; translation
<span lang="EN-US"><p>The determination of the amplitude of the power stroke produced by the elongation factor G-catalyzed ribosome translocation is an important issue, having strong implications for the molecular mechanism of the translocation. A recent paper (Yao et al., Angew. Chem. Int. Ed. 2013, 52, 14041 - 14044) tried to measure the amplitude of this power stroke. We show here that the explanation of the experimental data in the paper could be unreasonable and thus the amplitude of the power stroke determined there could be unreliable. We re-explain the experimental data and show that from these data the amplitude of the power stroke cannot be determined. Moreover, we give a different prediction of the amplitude of the power stroke.</p> </span>
Lorem Ipsum Press
2014-06-30 00:00:00
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/117
Journal of Molecular Biochemistry; Vol 3, No 2 (2014)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/116
2014-06-30T12:38:35Z
JmolBiochem:ART
"140630 2014 eng "
dc
Crystal structure and docking studies of hexahydrocycloocta[b]pyridine-3-carbonitriles
Vishnupriya, R
Kowsalyadevi, A.V.K.M.
Suresh, Janakiraman; Associate Professor
The Madura College
Maharani, S
Ranjith Kumar, R
Twisted boat Chair conformation, Intermolecular interactions, Chain motif, Antituberculosis activity, Molecular docking.
<p>The crystal structures of two new isomorphous pyridine structures, 2-ethoxy-4-(2-flurophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine-3-carbonitrile (Ia) and 2-methoxy-4-(4-isopropylphenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine-3-carbonitrile (Ib) were elucidated by single crystal X ray diffraction. Compound (Ia) C<sub>20</sub>H<sub>21</sub>FN<sub>2</sub>O, crystallizes in the monoclinic system, space group P2<sub>1</sub>/n<sub> </sub>with a = 7.0738 (3) Å, b = 17.3519(8) Å, c = 14.4239 (7) Å, b = 91.837 (2)° and Z = 4. The compound (Ib), C<sub>22</sub>H<sub>25</sub>N<sub>2</sub>O, crystallizes in the same crystal system as compound (Ib), space group P2<sub>1</sub>/c with a = 9.7123(6) Å, b = 20.6046(9) Å, c = 10.4657(6) Å, b = 117.208 (3)° and Z = 4. The central heterocyclic ring adopts a planar conformation and the cyclooctane ring adopts a twisted boat chair conformation in both (Ia) and (Ib). The synthesized compounds were screened for their anti-tuberculosis activity and were used to identify lead structures through docking studies, by automated docking. This approach was used to determine the orientation of inhibitors bound in the active site with the enzyme N-acetyl-gamma-glutamyl-phosphate reductase that is involved in arginine biosynthesis in M. tuberculosis (MtbAGPR). Details of the preparation, crystal structure determination, intra and inter molecular interactions of the compounds and their docking studies are given.</p>
Lorem Ipsum Press
2014-06-30 00:00:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/116
Journal of Molecular Biochemistry; Vol 3, No 2 (2014)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/122
2014-06-30T12:38:35Z
JmolBiochem:REV
"140630 2014 eng "
dc
Molecular dynamics simulations through GPU video games technologies
Loukatou, Styliani
Papageorgiou, Louis
Fakourelis, Paraskevas
Filntisi, Arianna
Polychronidou, Eleftheria
Bassis, Ioannis
Megalooikonomou, Vasileios
Makałowski, Wojciech
Vlachakis, Dimitrios
Kossida, Sophia
Molecular Dynamics; GPGPU; CUDA;
<p>Bioinformatics is the scientific field that focuses on the application of computer technology to the management of biological information. Over the years, bioinformatics applications have been used to store, process and integrate biological and genetic information, using a wide range of methodologies. One of the most de novo techniques used to understand the physical movements of atoms and molecules is molecular dynamics (MD).</p> <p>MD is an in silico method to simulate the physical motions of atoms and molecules under certain conditions. This has become a state strategic technique and now plays a key role in many areas of exact sciences, such as chemistry, biology, physics and medicine. Due to their complexity, MD calculations could require enormous amounts of computer memory and time and therefore their execution has been a big problem. Despite the huge computational cost, molecular dynamics have been implemented using traditional computers with a central memory unit (CPU).</p> <p>A graphics processing unit (GPU) computing technology was first designed with the goal to improve video games, by rapidly creating and displaying images in a frame buffer such as screens. The hybrid GPU-CPU implementation, combined with parallel computing is a novel technology to perform a wide range of calculations. GPUs have been proposed and used to accelerate many scientific computations including MD simulations. Herein, we describe the new methodologies developed initially as video games and how they are now applied in MD simulations.</p>
Lorem Ipsum Press
2014-06-30 00:00:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/122
Journal of Molecular Biochemistry; Vol 3, No 2 (2014)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/134
2014-11-03T23:20:15Z
JmolBiochem:ART
"141031 2014 eng "
dc
A series of Notch3 mutations in CADASIL; insights from 3D molecular modelling and evolutionary analyses
Vlachakis, Dimitrios
Champeris Tsaniras, Spyridon
Ioannidou, Katerina
Papageorgiou, Louis
Baumann, Marc
Kossida, Sophia
CADASIL; Notch3 mutations; Notch3 structure
<p class="AbstractText">CADASIL disease belongs to the group of rare diseases. It is well established that the Notch3 protein is primarily responsible for the development of CADASIL syndrome. Herein, we attempt to shed light to the actual molecular mechanism underlying CADASIL via insights that we have from preliminary in silico and proteomics studies on the Notch3 protein. At the moment, we are aware of a series of Notch3 point mutations that promote CADASIL. In this direction, we investigate the nature, extent, physicochemical and structural significance of the mutant species in an effort to identify the underlying mechanism of Notch3 role and implications in cell signal transduction. Overall, our in silico study has revealed a rather complex molecular mechanism of Notch3 on the structural level; depending of the nature and position of each mutation, a consensus significant loss of beta-sheet structure is observed throughout all in silico modeled mutant/wild type biological systems.</p>
Lorem Ipsum Press
2014-11-03 23:20:15
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/134
Journal of Molecular Biochemistry; Vol 3, No 3
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/127
2014-11-03T23:20:15Z
JmolBiochem:ART
"141031 2014 eng "
dc
Crystal structure and molecular docking studies of octahydrocycloocta[b]pyridine-3-carbonitriles as potential inhibitors against Mycobacterium tuberculosis
Nagalakshmi, RA; The Madura collge,
Madurai
Suresh, J; The Madura collge,
Madurai
Maharani, S; -
Ranjith Kumar, R
Conformation; Ring motif; Anti mycotuberculosis activity; Molecular docking; Inha
<p class="Default">The compounds 1-benzyl-2-imino-4-p-tolyl-1,2,5,6,7,8,9,10-octahydrocycloocta[b]pyridine-3-carbonitrile (Ia) and 1-benzyl-2-imino-(4-methoxyphenyl-1,2,5,6,7,8,9,10-octahydrocycloocta {b]pyridine-3-carbonitrile (Ib) were synthesized. The crystal structures of the compounds were determined by single crystal X ray diffraction. The compounds C<sub>26</sub> H<sub>27</sub> N<sub>3</sub> (Ia) and C<sub>26</sub> H<sub>27</sub> N<sub>3</sub>O (Ib) crystallize in the triclinic system (a = 10.2304(4) Å, b = 10.5655(4) Å, c = 11.8271(4) Å, α = 101.755(2) °, β = 106.934(2) °, γ = 114.071(2) ° and Z = 2 for I(a) and a = 10.2738(4) Å, b = 11.1654(5) Å, c = 11.4162(4) Å α = 98.549(2) °, β = 106.183(2) °, γ = 117.070(2) ° and Z = 2 for I(b)). In both compounds (Ia) and (Ib) the pyridine ring adopts a planar conformation and the cyclooctane ring adopts a twisted boat chair conformation. The synthesized compounds were screened for their antibacterial activities against the enzyme enoyl acyl carrier protein reductase, which is involved in the fatty acid biosynthesis of the mycobacterial cell wall. Both compounds showed good antibacterial activities. The synthesis of the compounds, their structure determination, their conformation, their intra- and intermolecular interactions and docking study results are given.</p> <p> </p><p class="Default"> </p>
Lorem Ipsum Press
2014-11-03 23:20:15
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/127
Journal of Molecular Biochemistry; Vol 3, No 3
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/130
2014-11-03T23:20:15Z
JmolBiochem:REV
"141031 2014 eng "
dc
SOCS, inflammation, and metabolism
Inagaki-Ohara, Kyoko; Research Institute, National Center for Global Health and Medicine (NCGM)
Yoshimura, Akihiko; Department of Microbiology and Immunology, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku, Tokyo, 160-8582, Japan
SOCS; obesity; cancer; leptin; insulin
<p>Obesity is characterized by the development of low-grade chronic inflammation, which is a contributing factor in defective energy metabolism. A hallmark of metabolic dysregulation, obesity is a life-style disease that contributes to diabetes, hypertension, and dyslipidemia. Further, recent studies warn that obesity can be a risk factor for certain cancers and exacerbates infectious diseases. This association is called the “metabolic domino”. Suppressor of cytokine signaling (SOCS) proteins are negative feedback regulators of cytokine and hormone signaling mediated by the JAK-STAT signaling pathway. SOCS proteins regulate cell-cell communication through JAK-STAT-dependent cytokines and signaling by Toll-like receptors (TLRs) and they may be influenced by dietary factors such as fatty acids and glucose. In this review, we focus on the role of the JAK-STAT-SOCS signaling cascade in metabolic disorder and obesity-related diseases.</p>
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Kyoko Inagaki-Ohara, Research Institute, National Center for Global Health and Medicine (NCGM), Akihiko Yoshimura, Department of Microbiology and Immunology, Keio University School of Medicine
2014-11-03 23:20:15
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/130
Journal of Molecular Biochemistry; Vol 3, No 3
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/133
2014-11-03T23:20:15Z
JmolBiochem:ED
"141031 2014 eng "
dc
Ebola virus epidemic: a deliberate accident?
Loukatou, Styliani
Fakourelis, Paraskevas
Papageorgiou, Louis
Megalooikonomou, Vasileios
Kossida, Sophia
Vlachakis, Dimitrios
ebola; epidemic; evd; ebov
Lorem Ipsum Press
2014-11-03 23:20:15
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/133
Journal of Molecular Biochemistry; Vol 3, No 3
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/135
2015-03-19T19:51:16Z
JmolBiochem:SC
"150315 2015 eng "
dc
GRP78 expression in canine mammary tumors: association with malignancy
de Oliveira, Joana T; Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal
Ribeiro, Cláudia
Gärtner, Fátima; IPATIMUP
GRP78; Mammary tumors; Canine; Microenvironment; Malignancy
<p>78-kDa glucose-regulated protein (GRP78) is over-expressed in human breast carcinomas. GRP78 expression was studied in 40 spontaneous canine mammary tumors and evaluated in relation to tumor histological type, mode of growth, grade, lymph node metastases and distant metastases. All tumors exhibited GRP78 immunostaining. In the normal canine mammary gland, GRP78 was also expressed although not in all cases. In carcinomas GRP78 was detected in the cytoplasm in more than 50% of tumor cells in the vast majority of cases (87.5%). There was a significant association between the absence of squamous differentiation (P = 0.02) and GRP78 over-expression, but no association with other clinico-pathological features. GRP78 was often co-expressed with galectin-3 in canine mammary tumors (CMT).</p> <p> </p>
Lorem Ipsum Press
2015-03-19 19:51:16
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/135
Journal of Molecular Biochemistry; Vol 4, No 1 (2015)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/140
2015-03-19T19:51:16Z
JmolBiochem:ART
"150315 2015 eng "
dc
Protein kinase CK1 interacts with and phosphorylates RanBPM in vitro
Wolff, Sonja; Department of General and Visceral Surgery
Surgery Center
University Hospital Ulm
Albert-Einstein-Allee 23
89081 Ulm
Germany
García-Reyes, Balbina; Department of General and Visceral Surgery
Surgery Center
University Hospital Ulm
Albert-Einstein-Allee 23
89081 Ulm
Germany
Henne-Bruns, Doris; Department of General and Visceral Surgery
Surgery Center
University Hospital Ulm
Albert-Einstein-Allee 23
89081 Ulm
Germany
Bischof, Joachim; Department of General and Visceral Surgery
Surgery Center
University Hospital Ulm
Albert-Einstein-Allee 23
89081 Ulm
Germany
Knippschild, Uwe; Department of General and Visceral Surgery
Surgery Center
University Hospital Ulm
Albert-Einstein-Allee 23
89081 Ulm
Germany
CK1; RanBPM; Yeast Two-Hybrid Screen; ran GTPase; phosphorylation; scaffold protein
<p>Members of the casein kinase 1 (CK1) family of serine/threonine kinases are highly conserved from yeast to mammals and are involved in the regulation of various cellular processes. Specifically, the CK1 isoforms δ and ε have been shown to be involved in the regulation of proliferative processes, differentiation, circadian rhythm, as well as in the regulation of nuclear transport. In this report we show that CK1δ and ε interact with murine RanBPM in the yeast two-hybrid system (YTH) and that the putative CK1δ-interacting domains of RanBPM are located between aa 155-386 and aa 515-653. Furthermore, in mammalian cells CK1δ partially co-localizes with RanBPM and can be co-immunoprecipitated with RanBPM. In addition, CK1δ strongly phosphorylates RanBPM within aa 436-514 <em>in vitro</em>. The identification of the interacting and scaffolding protein RanBPM as a new substrate of CK1δ points towards a possible function for CK1δ in modulating RanBPM specific functions.</p>
Lorem Ipsum Press
Grant from the Deutsche Krebshilfe, Dr. Mildred Scheel Stiftung, awarded to Uwe Knippschild (108489
2015-03-19 19:51:16
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/140
Journal of Molecular Biochemistry; Vol 4, No 1 (2015)
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Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/141
2015-03-19T19:51:16Z
JmolBiochem:ED
"150315 2015 eng "
dc
Optimisation of a potent series of HCV helicase drug candidates
Loukatou, Styliani
Papageorgiou, Louis
Vlachakis, Dimitrios
Lorem Ipsum Press
2015-03-19 19:51:16
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/141
Journal of Molecular Biochemistry; Vol 4, No 1 (2015)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/142
2015-07-20T10:59:17Z
JmolBiochem:ART
"150719 2015 eng "
dc
Human hair follicle biomagnetism: potential biochemical correlates
Embi, Abraham A; Independent-Retired-
Associated with The University of Oklahoma, Oklahoma City, OK USA
Scherlag, Benjamin J
biomagnetism; S100; electromagnetic field; hair follicle
<p>Background: The S100 protein family is linked to energy transfer in cells of vertebrates at a molecular level. This process involves the electron transfer chain and therefore, as inferred from Faraday’s Law, electron movement will induce electromagnetic fields (EMFs). Biological entities emit photoelectrons that can be tracked and visualized by small paramagnetic nano-sized iron particles.</p> <p>Methods: We have developed an optical microscopic approach for imaging electromagnetic activity of hair follicles utilizing nano-sized iron particles (mean diameter 2000nm) in Prussian Blue Stain solution (PBS Fe 2000).</p> <p>Results: We found that the human hair follicle emits electromagnetic fields (EMFs) based on metabolic activity within the follicle, which is associated with the activity of selective S-100 proteins.</p> <p>Conclusions: Our results link the molecular biochemical energy associated with the S100 family of proteins and biomagnetism of human hair follicles.</p>
Lorem Ipsum Press
2015-07-20 10:59:17
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/142
Journal of Molecular Biochemistry; Vol 4, No 2 (2015)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/142/496
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/142/497
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/143
2015-07-20T10:59:17Z
JmolBiochem:ART
"150719 2015 eng "
dc
Antimicrobial activities of caffeic acid phenethyl ester
Meyuhas, Sivan
Assali, Morad
Huleihil, Mahmoud
Huleihel, Mahmoud
CAPE; Propolis; gram (+) bacteria; gram (-) bacteria; antibacterial effect
<p>Caffeic acid phenethyl ester (CAPE) is considered as one of the most active components of Propolis extract (PE), a natural product obtained from beehives. PE comprises a complex of chemicals and has been found to have various biological activities. The aim of the present study is to assess the antibacterial activities CAPE has against various gram positive [gram (+)] and gram negative [gram (-)] bacteria and try to elucidate its mechanism of action. Bacteria were grown in the presence of various doses of CAPE and examined at different periods of time for their growth, both by absorbance (OD) measurement and colony assay. The results show that CAPE significantly inhibited the growth of most examined gram (+) bacteria while having only a slight inhibitory effect on most tested gram (-) bacteria. Our results also show that continuous treatment of gram (+) bacteria with CAPE for at least 6h caused irreversible inhibition of the bacterial growth (bacteriocidic effect); however, treatment for shorter periods of time caused only a stopping of bacterial growth (bacteriostatic effect). It seems that these effects were caused, at least partially, as a result of disruptions of the treated bacterial outer and plasma membranes. There is no significant synergistic effect between CAPE and ampicillin, although an additive effect has been found.</p> <p> </p><p> </p>
Lorem Ipsum Press
2015-07-20 10:59:17
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/143
Journal of Molecular Biochemistry; Vol 4, No 2 (2015)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/145
2015-07-20T10:59:17Z
JmolBiochem:REV
"150719 2015 eng "
dc
Protein phosphorylation prediction: limitations, merits and pitfalls
Vlachakis, Dimitrios; Bioinformatics & Medical Informatics Team, Biomedical Research Foundation, Academy of Athens, Soranou Efessiou 4, Athens 11527
Bencurova, Elena; Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 04181, Kosice
Papageorgiou, Louis; Bioinformatics & Medical Informatics Team, Biomedical Research Foundation, Academy of Athens, Soranou Efessiou 4, Athens 11527
Department of Informatics and Telecommunications, National and Kapodistrian University of Athens, University Campus, Athens, 15784
Bhide, Mangesh; Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 04181
Institute of Neuroimmunology, Slovak Academy of Sciences, 84245 Bratislava
Kossida, Sophia; Bioinformatics & Medical Informatics Team, Biomedical Research Foundation, Academy of Athens, Soranou Efessiou 4, Athens 11527, Greece
IMGT®, the international ImMunoGeneTics information system®, Institute of Human Genetics, Montpellier, France
Bioinformatics; Protein Phosphorylation; Post-Translational Modifications; Benchmark; Phosphorylation Prediction; GPS; NetPhos; Phospho.ELM; PPSP; SMALI; ScanSite; Musite; NetPhos
<p>Protein phosphorylation is a major protein post-translational modification process that plays a pivotal role in numerous cellular processes, such as recognition, signaling or degradation. It can be studied experimentally by various methodologies, including western blot analysis, site-directed mutagenesis, 2D gel electrophoresis, mass spectrometry etc. A number of in silico tools have also been developed in order to predict plausible phosphorylation sites in a given protein. In this review, we conducted a benchmark study including the leading protein phosphorylation prediction software, in an effort to determine which performs best. The first place was taken by GPS 2.2, having predicted all phosphorylation sites with a 83% fidelity while in second place came NetPhos 2.0 with 69%.</p> <p> </p><p> </p>
Lorem Ipsum Press
2015-07-20 10:59:17
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/145
Journal of Molecular Biochemistry; Vol 4, No 2 (2015)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/145/503
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/145/504
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/149
2015-07-20T10:59:17Z
JmolBiochem:ART
"150719 2015 eng "
dc
Tumour growth in mice resistant to diet-induced obesity
Dachs, Gabi U; University of Otago, Christchurch
Phillips, Elisabeth
Phung, Yen
Dyer, Arron
Willis, Jinny A
Currie, Margaret J
Robinson, Bridget A
mouse model; BALB/c; syngeneic tumour; Western diet; obesity; colorectal cancer
<p>Obesity is a chronic disease with associated increases in the incidence, and a reduction in survival, of many cancer types. Obesity results from an imbalance in calorie intake and calorie requirement. This study aimed to investigate the separate effects of high-fat diet and obesity on cancer in an animal model resistant to diet-induced obesity. Male BALB/c mice fed long-term on a high-fat, Western-style diet were implanted with syngeneic CT26 colon adenocarcinoma cells and compared to mice fed normal diet. BALB/c mice on high-fat diet were 10% heavier than mice fed normal diet, with no difference in tumour growth rates or tumour cell proliferation. Subgroups of mice that became obese on high-fat diet, however, showed increased tumour growth rates compared to mice fed normal diet, whereas mice that remained slim showed no difference in tumour growth. Protein arrays identified several adipokines that were expressed at different levels, including serum Tissue Inhibitors of Metallo-Proteinases (TIMP-1) and tumour C-Reactive Protein (CRP). In conclusion, tumour growth was enhanced in mice unable to resist obesity, and adipokine profiles were affected by the animals’ ability to resist obesity.</p>
Lorem Ipsum Press
Genesis Oncology Trust; Mackenzie Charitable Foundation
2015-07-20 10:59:17
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/149
Journal of Molecular Biochemistry; Vol 4, No 2 (2015)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/151
2015-07-20T10:59:17Z
JmolBiochem:ED
"150719 2015 eng "
dc
Diet, obesity and cancer
Champeris Tsaniras, Spyridon
Vlachakis, Dimitrios
diet; obesity; cancer
Lorem Ipsum Press
2015-07-20 10:59:17
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/151
Journal of Molecular Biochemistry; Vol 4, No 2 (2015)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/167
2015-11-27T19:50:58Z
JmolBiochem:ART
"151127 2015 eng "
dc
Cell cycle-dependent phosphorylation of nucleophosmin and its potential regulation by peptidyl-prolyl cis/trans isomerase
Zhao, Xuelian
Ji, Junfang
Yu, Li-Rong
Veenstra, Timothy
Wang, Xin Wei
nucleophosmin; Pin1; cell cycle; phosphorylation
<p>Nucleophosmin (NPM) is a ubiquitously expressed phosphoprotein involved in many cellular processes. Phosphorylation is considered the major regulatory mechanism of the NPM protein, associated with diverse cellular events. In this study, we characterized the phosphorylation status of several physiological phosphorylation sites of NPM, especially the newly confirmed <em>in vivo</em> site threonine 95 (Thr95). NPM-Thr95 exhibits a transient and cell cycle-dependent phosphorylation state compared to several other <em>in vivo</em> phosphorylation sites examined, including Ser4, Thr199 and Thr234/Thr237. In addition, we characterized a functional interaction between NPM and the peptidyl-prolyl isomerase Pin1, which specifically bind to each other during mitosis. The demonstration of this binding represents a novel post-phosphorylation regulatory mechanism for NPM that has not been investigated before. Mutated Pin1 putative binding sites result in defected cell division and reduced number of mitotic cells, suggesting that post-phosphorylation is important for NPM in regulating cell cycle progression.</p>
Lorem Ipsum Press
National Cancer Institute
2015-11-27 19:50:59
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/167
Journal of Molecular Biochemistry; Vol 4, No 3 (2015)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/169
2015-11-27T19:50:58Z
JmolBiochem:ED
"151127 2015 eng "
dc
The Nucleophosmin-Pin1 interaction links the cell cycle, cancer and pluripotency
Champeris Tsaniras, Spyridon
Vlachakis, Dimitrios
Taraviras, Stavros
nucleophosmin, pin1, cell cycle, pluripotency, cancer
Lorem Ipsum Press
2015-11-27 19:50:59
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/169
Journal of Molecular Biochemistry; Vol 4, No 3 (2015)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/148
2015-11-27T19:50:59Z
JmolBiochem:ART
"151127 2015 eng "
dc
Resveratrol-fortification of red wine does not provide greater inhibition of human lung cancer cell survival compared to non-fortified wine.
Moore, Jessy; Brock University
Pickering, Gary; Brock University
Gaudette, Nicole J; Brock University
Tsiani, Evangelia; Brock University
cancer, survival, Akt, ERK, red wine, resveratrol
<p>Lung cancer is the leading cause of cancer-related deaths, and individuals with this disease often develop resistance to conventional cytotoxic therapies. Red wine and its polyphenolic component resveratrol, have been shown to have anticancer effects. Wines fortified with resveratrol have been marketed as having additional health benefits because of their increased polyphenolic content, however no studies exist examining this claim. The aim of the present study was to explore the effects of resveratrol-fortified red wine on lung cancer cell survival. Human NSCLC A549 cells were treated with varying concentrations of red wine with or without <em>trans</em>-resveratrol fortification. Cell survival was assessed using clonogenic assays and immunoblotting was used to explore the effects on Akt and ERK signaling molecules. Red wine significantly inhibited cell survival at concentrations as low as 0.02%, and significantly reduced phosphorylation of both Akt and ERK. No significant differences were seen between regular and resveratrol-fortified red wine. These data suggest that red wine may have considerable cancer preventive potential, however it does not support the use of resveratrol-fortified wine for additional health benefits.</p><p> </p>
Lorem Ipsum Press
2015-11-27 19:50:59
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/148
Journal of Molecular Biochemistry; Vol 4, No 3 (2015)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/150
2015-11-27T19:50:59Z
JmolBiochem:REV
"151127 2015 eng "
dc
Biochemical and cytogenetic changes in postovulatory and in vitro aged mammalian oocytes: a predisposition to aneuploidy
Mailhes, John B.
postovulatory ageing; oocyte; aneuploidy; premature centromere separation
<p>Aneuploidy represents the most prevalent genetic disorder of man. Its association with spontaneous abortions, mental and physical retardation, and numerous malignant cells is well-known. Unfortunately, little is known about the causes and even less about the underlying molecular mechanisms of aneuploidy, especially in mammalian germ cells. Although several etiologies have been proposed for describing human aneuploidy, the only consistent finding remains its positive correlation with maternal age. At the outset, it is essential to point out that there exist numerous potential causes and mechanisms for the etiology of aneuploidy. Nevertheless, information about the molecular mechanisms of chromosome segregation in various species is providing a foundation for research designed to investigate the causes and mechanisms of aneuploidy. The intent of this review is to propose that the biochemical reactions and cellular organelles responsible for accurate chromosome segregation become compromised during postovulatory and in vitro oocyte aging; thus, increasing the probability of faulty chromosome segregation. Recent data have shown that the efficacies of the spindle assembly checkpoint and the chromosome cohesion proteins diminish as oocytes age postovulation and during in vitro culture. Such changes represent potential models for studying aneuploidy. Prior to describing the biochemical and cellular organelle changes found in aged oocytes and their effect on chromosome segregation, an overview of the molecular details surrounding chromosome segregation is presented.</p> <p> </p><p> </p>
Lorem Ipsum Press
2015-11-27 19:50:59
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/150
Journal of Molecular Biochemistry; Vol 4, No 3 (2015)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/190
2016-03-31T09:13:29Z
JmolBiochem:ED
"160330 2016 eng "
dc
HCV, Dengue, West Nile and now Zika; What’s next? The threat of endemic TBE relatives in Southern Europe
Vlachakis, Dimitrios
Champeris Tsaniras, Spyridon
Flaviviridae; Zika; TBE; Tick-borne encephalitis; GGE; Greek Goat Encephalitis
Lorem Ipsum Press
2016-03-31 09:13:29
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/190
Journal of Molecular Biochemistry; Vol 5, No 1 (2016)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/178
2016-03-31T09:13:29Z
JmolBiochem:ART
"160330 2016 eng "
dc
High-throughput cell-based compound screen identifies pinosylvin methyl ether and tanshinone IIA as inhibitors of castration-resistant prostate cancer
Ketola, Kirsi; Turku Centre for Biotechnology, University of Turku
Viitala, Miro; Turku Centre for Biotechnology, University of Turku
Kohonen, Pekka; Medical Biotechnology, VTT Technical Research Centre of Finland, Finland
Fey, Vidal; Medical Biotechnology, VTT Technical Research Centre of Finland, Finland
Culig, Zoran; Division of Experimental Urology, Innsbruck Medical University, Innsbruck, Austria
Kallioniemi, Olli; Institute for Molecular Medicine, Finland (FIMM), University of Helsinki, Finland
Iljin, Kristiina; Medical Biotechnology, VTT Technical Research Centre of Finland, Finland
Prostate Cancer; High-throughput screen; PSME; tanshinone IIA; CRPC
<p>Current treatment options for castration-resistant prostate cancer (CRPC) are limited. In this study, a high-throughput screen of 4910 drugs and drug-like molecules was performed to identify antiproliferative compounds in androgen ablated prostate cancer cells. The effect of compounds on cell viability was compared in androgen ablated LNCaP prostate cancer cells and in LNCaP cells grown in presence of androgens as well as in two non-malignant prostate epithelial cells (RWPE-1 and EP156T). Validation experiments of cancer specific anti-proliferative compounds indicated pinosylvin methyl ether (PSME) and tanshinone IIA as potent inhibitors of androgen ablated LNCaP cell proliferation. PSME is a stilbene compound with no previously described antineoplastic activity whereas tanshinone IIA is currently used in cardiovascular disorders and proposed as a cancer drug. To gain insights into growth inhibitory mechanisms in CRPC, genome-wide gene expression analysis was performed in PSME- and tanshinone IIA-exposed cells. Both compounds altered the expression of genes involved in cell cycle and steroid and cholesterol biosynthesis in androgen ablated LNCaP cells. Decrease in androgen signalling was confirmed by reduced expression of androgen receptor and prostate specific antigen in PSME- or tanshinone IIA-exposed cells. Taken together, this systematic screen identified a novel anti-proliferative agent, PSME, for CRPC. Moreover, our screen confirmed tanshinone IIA as well as several other compounds as potential prostate cancer growth inhibitors also in androgen ablated prostate cancer cells. These results provide valuable starting points for preclinical and clinical studies for CRPC treatment.</p>
Lorem Ipsum Press
Marie Curie Canceromics (MEXT-CT-2003-2728), EU-PRIMA project (contract # LSHC-CT-204-504587), EU-GENICA project (FP7-HEALTH-2007-A), Academy of Finland, the Cancer Organizations of Finland and Sigrid Juselius stiftelse
2016-03-31 09:13:29
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/178
Journal of Molecular Biochemistry; Vol 5, No 1 (2016)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/178/566
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/178/567
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/178/568
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/182
2016-03-31T09:13:29Z
JmolBiochem:ART
"160330 2016 eng "
dc
Sensitization of human melanoma cells for TRAIL-induced apoptosis by a selective aurora kinase A inhibitor
Geilen, Cyprienne Isabell; Charité-Universitätsmedizin Berlin
Dermatology, Venerology and Allergy
Skin Cancer Center
Plötz, Michael; Charité-Universitätsmedizin Berlin
Dermatology, Venerology and Allergy
Skin Cancer Center
Quast, Sandra Annika; Charité-Universitätsmedizin Berlin
Dermatology, Venerology and Allergy
Skin Cancer Center
Steinhorst, Katja; Charité-Universitätsmedizin Berlin
Dermatology, Venerology and Allergy
Skin Cancer Center
Geilen, Christoph C.; 1. Charité-Universitätsmedizin Berlin
Dermatology, Venerology and Allergy
Skin Cancer Center
2. Faculty of Human Sciences, Medical School Hamburg, Hamburg, Germany
Eberle, Jürgen; Charité-Universitätsmedizin Berlin
Dermatology, Venerology and Allergy
Skin Cancer Center
Melanoma; Apoptosis; TRAIL; Aurora kinase; Alisertib
<p>Different therapeutic strategies in metastatic melanoma focused on signalling pathways controlling cell proliferation, cell cycle and apoptosis. While TRAIL (TNF-related apoptosis inducing ligand) has been shown to be an interesting candidate for inducing apoptosis in cancer cells without affecting normal cells, the ability of cancer cells to develop resistance, limits its therapeutic potential. Using a recently established experimental A-375 melanoma cell model for investigating TRAIL resistance, we could demonstrate that the aurora kinase A inhibitor Alisertib (MLN 8237) enhances the proapoptotic effects of TRAIL and sensitizes TRAIL-selected melanoma cells with acquired resistance, associated with an activation of intrinsic mitochondrial apoptotic pathways. In course of this activation an upregulation of p53 in the nuclear fraction was shown. Thus, the aurora kinase A inhibitor Alisertib is able to overcome TRAIL-induced resistance in melanoma cells suggesting the combination of TRAIL and Alisertib as a promising therapeutic strategy for metastatic melanoma.</p> <p> </p>
Lorem Ipsum Press
2016-03-31 09:13:29
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/182
Journal of Molecular Biochemistry; Vol 5, No 1 (2016)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/144
2016-03-31T09:13:29Z
JmolBiochem:ART
"160330 2016 eng "
dc
The effects of cystatin C construct clones on B16F10 in vitro cell behavior
Cox, James Lewis; AT Still University
McIntire, Seth; ATSU
cystatin; metastasis; melanoma; migration; B16F10
<p><strong>Background:</strong> Metastasis is the cause of most cancer related morbidity. A naturally occurring cysteine protease inhibitor, cystatin C, has been reported to inhibit tumor cell metastasis for several different cancer types, however, the mechanism is still unknown. Our study focused on determining which region of cystatin C is responsible for anti-metastatic action by characterizing specific constructs of cystatin C in melanoma cells. In one construct, the N-terminal peptide amino acids 1-10, required for cysteine protease inhibition, were deleted. In the second construct the conserved motif QLVA was altered to GGGG.<strong> Methods and Results:</strong> Net proliferation, migration and invasion of the cystatin C constructs were assessed. The modified Boyden chamber revealed 75% reduced invasion of N-truncated clones compared to control B16F10, similar to cystatin C overexpression. A scratch migration assay also showed a three-fold reduction in migration rate. The QLVA sequence was found to be required for inhibition of B16F10 invasion and migration. Net cell proliferation remained constant between clones. <strong>Conclusions:</strong> Overexpression of cystatin C with or without cysteine protease inhibitor activity inhibits the migration and invasion, but not proliferation, of B16F10 melanoma cells. The conserved cystatin sequence, QLVA, is required for cystatin actions on B16 melanoma cells.</p>
Lorem Ipsum Press
2016-03-31 09:13:29
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/144
Journal of Molecular Biochemistry; Vol 5, No 1 (2016)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/175
2016-08-01T16:12:29Z
JmolBiochem:ART
"160731 2016 eng "
dc
Crystal structure and molecular docking studies of benzo[8]annulenes as potential inhibitors against Mycobacterium tuberculosis
Nagalakshmi, RA; The Madura collge,
Madurai
Suresh, J; The madura college,
madurai
Maharani, S; Madurai Kamraj university,
Madurai
Kumar, R Ranjith; Madurai kamaraj university,
Madurai
conformation; ring motif; anti-mycobacterial; molecular docking; mycobacterium tuberculosis; benzo[8]annulenes; crystal structure
<p>Tuberculosis is a disease caused by Mycobacterium tuberculosis. The bacterial cell wall has a characteristic low permeability, which essentially makes antibiotics ineffective. The cell wall material must be regulated so that its deposition does not compromise its structure. In this study, two new inhibitors, 2-amino-4-(4-cholorophenyl)-5,6,7,8,9,10-hexahydrobenzo[8] annulene-1,3,3(4H)-tricarbonitrile(Ia) and 2-amino-4-(4-bromophenyl)-5,6,7,8,9,10-hexahydrobenzo[8]annulene-1,3,3(4H)-tricarbonitrile(Ib) were synthesized. The crystal structures of the above compounds were determined by single crystal X-ray diffraction. The compounds C<sub>21</sub> H<sub>19 </sub>Cl N<sub>3</sub> (Ia) and C<sub>21</sub> H<sub>19 </sub>Br N<sub>3</sub> (Ib) were crystallized in the monoclinic and triclinic system. In both compounds, the cyclohexane ring was found to adopt a boat conformation. The cyclooctane ring of both compounds adopted a twisted chair-chair conformation. In silico analyses revealed that both compounds showed good anti-mycobacterial activities against the enoyl-acyl carrier enzyme and the N-acetyl-gamma protein, both of which are critical for bacterial survival. Synthesis, structure determination, conformation, intra, inter-molecular interactions and docking studies of both compounds are presented herein.</p>
Lorem Ipsum Press
2016-08-01 16:12:29
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/175
Journal of Molecular Biochemistry; Vol 5, No 2 (2016)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/194
2016-08-01T16:12:29Z
JmolBiochem:ART
"160731 2016 eng "
dc
The effectiveness of a stress-management intervention program in the management of overweight and obesity in childhood and adolescence
Stavrou, Stavroula
Nicolaides, Nicolaos; Aglaia Kyriakou Hospital
Papageorgiou, Ifigenia
Papadopoulou, Pinelopi
Terzioglou, Elena
Chrousos, George P
Darviri, Christina
Charmandari, Evangelia
stress; stress management; childhood obesity
<p>Background: Obesity in childhood and adolescence represents a major health problem of our century, and accounts for a significant increase in morbidity and mortality in adulthood. In addition to the increased consumption of calories and lack of exercise, accumulating evidence suggests that childhood obesity is strongly associated with prolonged and excessive activation of the stress system.</p> <p>Aim: The aim of our study was to assess the effectiveness of a stress-management intervention program, which included progressive muscle relaxation, diaphragmatic breathing, guided imagery and cognitive restructuring, in overweight and obese children and adolescents.</p> <p>Methods: Forty-nine children and adolescents (mean age ± SEM: 11.15 ± 1.48 years) were prospectively recruited to participate in this randomized controlled study. Of those, 23 participants were assigned into the intervention group, while 26 participants represented the control group. Anthropometric measurements were recorded at the beginning and at the end of the study, and participants were asked to complete the Screen for Child Anxiety Related Disorders (S.C.A.R.E.D.), the Child Depression Inventory (C.D.I.), the Child Behavior Checklist (C.B.C.L.) and the Youth Self Report (Y.S.R.).</p> <p>Results: The applied stress-management methods resulted in a significant reduction in the body mass index (BMI) in the intervention group compared with the control group [ΔBMI=1.18 vs 0.10 kg/m<sup>2</sup> (p˂0.001)]. In addition to BMI, these methods ameliorated depression and anxiety, and reduced the internalizing and externalizing problems in the intervention group.</p> <p>Conclusions: Our study demonstrated that the application of an 8-week stress management program could facilitate weight loss in Greek overweight and obese children and adolescents. Further larger studies are required to evaluate the effectiveness of stress-management methods in overweight and obese subjects.</p>
Lorem Ipsum Press
2016-08-01 16:12:29
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/194
Journal of Molecular Biochemistry; Vol 5, No 2 (2016)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/185
2016-08-01T16:12:29Z
JmolBiochem:ART
"160731 2016 eng "
dc
Study on the internalization mechanism of the ZEBRA cell-penetrating peptide
Marchione, Roberta
Liguori, Lavinia
Laurin, David
Lenormand, Jean-Luc; HumProTher, TheReX Laboratory, TIMC-IMAG, UMR 5525, School of Pharmacy, Building Jean Roget, Domaine de la Merci, 38700 La Tronche France
ZEBRA; Cell-Penetrating Peptide; transduction domain; endocytosis; endosomal escape
<p>Cell-penetrating peptides (CPPs) represent a noninvasive method for delivering functional biomolecules into living cells. We have recently shown that the Epstein-Barr virus transcriptional factor ZEBRA contains a protein transduction domain, named Z9 or minimal domain (MD). Only few of currently identified CPPs including MD are able to rapidly cross the mammalian cell membrane without being entrapped into endosomal compartments, even when fused to cargo macromolecules. In this work, a series of MD deletion mutants has been engineered and their cellular uptake has been analyzed by confocal microscopy and FACS. We identified a domain MD<sub>11</sub> (8 amino acids shorter than MD) able to enter mammalian cells via a mainly endocytosis-independent mechanism. All the other generated truncated forms exhibited reduced cellular uptake and penetrated into cells through endocytic mechanisms. These results have highlighted the role of the MD<sub>11</sub> C-terminal region as essential for efficient cellular entry and endosomal escape and open new perspectives for the use of this CPP as carrier for delivering biologically active macromolecules with therapeutic potential.</p>
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GEFLUC
2016-08-01 16:12:29
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/185
Journal of Molecular Biochemistry; Vol 5, No 2 (2016)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/189
2016-08-01T16:12:29Z
JmolBiochem:ART
"160731 2016 eng "
dc
Increased transgene expression mediated by recombinant adeno-associated virus in human neuroglia cells under microgravity conditions
Sun, Feiyi; Beijing Institute of Technology
Zhang, Jiewen; Beijing Institute of Technology
Ma, Chengwei; Beijing Institute of Technology
Zhang, Yaxi; Beijing Institute of Technology
Li, Yali; Xuan-wu hospital of Capital Medical University
Zhang, Lan; Xuan-wu hospital of Capital Medical University
Deng, Yulin; Beijing Institute of Technology
Ma, Hong; Beijing Institute of Technology
microgravity; Adeno-associated virus; adhesion ability; gene expression; pathogenicity
<p>The space environment has the special characteristics of radiation, noise particularity and weightlessness, all of which have adverse effects on astronauts’ muscles, bones, neurons and immune system. Some reports have shown that chemotherapy and radiotherapy can increase the activity of the recombinant adeno-associated virus (AAV) which is widely used in gene therapy. In this paper, recombinant AAV2 (rAAV2) was first packaged with the enhanced green fluorescence protein (eGFP) gene and used to infect neuroglia cells including the U87 and U251 cell lines, under microgravity conditions; it was then detected by fluorescence microscopy and flow cytometry. The results show that microgravity affects the adhesion ability of cells, promotes transgene expression induced by rAAV2 and causes changes of viral infection receptors at different time points. These findings broaden the current understanding of the microgravity effects on rAAV, with significant implications in gene therapy and the mechanisms of increased virus pathogenicity under space microgravity.</p><p> </p>
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National Natural Science Foundation of China;Beijing Institute of Technology;Xuan-wu hospital of Capital Medical University
2016-08-01 16:12:29
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/189
Journal of Molecular Biochemistry; Vol 5, No 2 (2016)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/196
2016-08-01T16:12:29Z
JmolBiochem:ED
"160731 2016 eng "
dc
Beyond diet and exercise: Stress management as a novel promising strategy for childhood and adolescent obesity
Chrousos, George P
childhood obesity; adolescent obesity; stress management; BMI reduction
Lorem Ipsum Press
2016-08-01 16:12:29
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/196
Journal of Molecular Biochemistry; Vol 5, No 2 (2016)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/195
2016-11-29T17:23:32Z
JmolBiochem:ART
"161129 2016 eng "
dc
Cloning and functional characterization of a vertebrate low-density lipoprotein receptor homolog from eri silkmoth, Samia ricini
Bala, Rajni
Saba, Ulfath
Varma, Meenakshi
Thomas, Dyna Susan
Sinha, Deepak Kumar
Rao, Guruprasad
Trivedy, Kanika
Kunjupillai, Vijayan
Gopalapillai, Ravikumar; Seri-biotech Research Laboratory
low-density lipoprotein receptor, lipophorin receptor, eri silkmoth, RNAi, ligand binding, cross reactivity.
<p>The lipophorin receptor (LpR) is the insect lipoprotein receptor and belongs to the low-density lipoprotein receptor (LDLR) superfamily. It has a vital role in the uptake of lipophorin (Lp) into various tissues. Here we report the full length cloning and functional characterization of an LpR from eri silkmoth, <em>Samia ricini</em>. The full length cDNA of SrLpR7-1 is 4132 bp including an open reading frame (ORF) of 2595 bp. The deduced amino acid sequence revealed well structured ligand binding, epidermal growth factor, glycosylation, transmembrane and cytoplasmic domains. The ligand binding domain consisted of seven cysteine repeats instead of the common eight cysteine repeats indicating it as a homolog of human LDLR. We identified another splice variant, <em>Sr</em>LpR7-2 with a deletion of 27 amino acids in the O-glycosylation domain. Apart from the fat body, both isoforms are expressed in ovary, brain and other tissues at different developmental stages of the silkworm. RNAi experiments did not show any marked effects except that the adult emergence was delayed compared to controls. In addition, the SrLpR7 cDNA was recombinantly expressed and ligand binding experiments confirmed that the receptor protein binds not only to <em>Sr</em>Lp but also to <em>Bombyx mori</em> Lp.</p>
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Department of Biotechnology
2016-11-29 17:23:32
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/195
Journal of Molecular Biochemistry; Vol 5, No 3 (2016)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/183
2016-11-29T17:23:32Z
JmolBiochem:ART
"161129 2016 eng "
dc
Antioxidant and antiproliferative activities of methanolic extracts of beer
Mancinelli, Loretta; University of Perugia (Italy)
Del Pino, Alberto Marco; University of Perugia, Italy
Mancini, Francesco; University of Perugia, Italy
Belia, Silvia; University of Perugia,Italy
Palmerini, Carlo Alberto; University of Perugia, Italy
Mazzoni, Michela; University of Perugia, Italy
Granieri, Letizia; University of Perugia, Italy
Perretti, Giuseppe; University of Perugia, Italy
beer; antioxidants; cell growth; ROS; anticancer
<p>The beneficial effects of beer on human health, such as reduced risk of heart diseases and certain types of cancer, has been associated to its polyphenolic content and resulting antioxidant activity. The aim of this study was to verify the relationship between the antioxidant and antiproliferative activity of the compounds contained in beer. In order to evaluate different antioxidant profiles, methanolic extracts of various commercial beers and of samples collected during the brewing process were analyzed. Their antioxidant power was evaluated by measuring: i) the ferric reducing activity, ii) their ability in suppressing the release of thiobarbituric acid reactive substances and iii) the content of antioxidant compounds. The antiproliferative activity was detected by determining the inhibition of HeLa cancer cell growth following incubation with the extracts. The results of this study showed that most beer extracts have significant antiproliferative effects, but these effects were not due to the antioxidant power of the extracts. By contrast, they were found to be related to the extract-induced increase of the intracellular levels of reactive oxygen species. The antiproliferative action of the extracts could result from a pro-oxidant mechanism due to non–phenolic compounds.</p>
Lorem Ipsum Press
2016-11-29 17:23:32
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/183
Journal of Molecular Biochemistry; Vol 5, No 3 (2016)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/183/577
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/183/578
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/183/579
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/183/580
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/183/581
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/183/583
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/187
2016-11-29T17:23:32Z
JmolBiochem:ART
"161129 2016 eng "
dc
Identification of microRNA-365 as a negative regulator of endothelial cell proliferation
Wu, Xiao; Shandong University
Jiang, Fan; Shandong University
microRNA; miR-365; vascular endothelial cell; proliferation; serum- and glucocorticoid-regulated kinase-1; microvesicle
<p>Recent studies have shown that human microRNA miR-365 has significant inhibitory effects on proliferation of transformed cancer cells and vascular smooth muscle cells. However, the effects of miR-365 on proliferation and migration of vascular endothelial cells remain unknown. Using human umbilical vein endothelial cells and in vitro assays, we demonstrated that miR-365 was a suppressor of endothelial cell proliferation, whereas cell migration was not affected by miR-365. We also identified that the expression level of the serine/threonine protein kinase serum- and glucocorticoid-regulated kinase-1 (SGK-1) was regulated by miR-365. The cytostatic effect of miR-365 was mimicked by the specific SGK-1 inhibitor GSK 650394. We further demonstrated that microvesicles isolated from plasma of patients with intracerebral hemorrhage, in which the level of miR-365 was elevated, decreased the expression level of SGK-1, and this effect was abolished in cells pretreated with miR-365 antagomir. However, we did not observe a significant effect of the microvesicles on cell proliferation. It is suggested that miR-365 may have important roles in vascular physiology and/or pathophysiology by modulating endothelial cell proliferation.</p><p> </p>
Lorem Ipsum Press
Natural Science Foundation China
2016-11-29 17:23:32
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/187
Journal of Molecular Biochemistry; Vol 5, No 3 (2016)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/199
2017-04-26T11:00:58Z
JmolBiochem:ART
"170422 2017 eng "
dc
The Role of S-Palmitoylation of the Human Glucocorticoid Receptor (hGR) in Mediating the Nongenomic Glucocorticoid Actions
Nicolaides, Nicolaos; Aglaia Kyriakou Hospital
Kino, Tomoshige
Roberts, Michael L
Katsantoni, Eleni
Sertedaki, Amalia
Moutsatsou, Paraskevi
Psarra, Anna-Maria G.
Chrousos, George P
Charmandari, Evangelia
hGR; Human Glucocorticoid Receptor; S-Palmitoylation
<p>Background: Many rapid nongenomic glucocorticoid actions are mediated by membrane-bound glucocorticoid receptors (GRs). S-palmitoylation is a lipid post-translational modification that mediates the membrane localization of some steroid receptors. A highly homologous amino acid sequence (663YLCM KTLLL671) is present in the ligand-binding domain of hGRα, suggesting that hGRα might also undergo S-palmitoylation.</p> <p>Aim: To investigate the role of the motif 663YLCMKTLLL671 in membrane localization of the hGRα and in mediating rapid nongenomic glucocorticoid signaling.</p> <p>Methods and Results: We showed that the mutant receptors hGRαY663A, hGRαC665A and hGRαLL670/671AA, and the addition of the palmitoylation inhibitor 2-bromopalmitate did not prevent membrane localization of hGRα and co-localization with caveolin-1, and did not influence the biphasic activation of mitogen-activated protein kinase (MAPK) signaling pathway in the early time points. Finally, the hGRα was not shown to undergo S-palmitoylation.</p> <p>Conclusions: The motif 663YLCMKTLLL671 does not play a role in membrane localization of hGRα and does not mediate the nongenomic glucocorticoid actions.</p> <p> </p><p> </p>
Lorem Ipsum Press
2017-04-26 11:00:58
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/199
Journal of Molecular Biochemistry; Vol 6, No 1 (2017)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/199/708
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/199/709
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/207
2017-04-26T11:00:58Z
JmolBiochem:ART
"170422 2017 eng "
dc
HCMV activation of ERK-MAPK drives a multi-factorial response promoting the survival of infected myeloid progenitors
Kew, Verity
Wills, Mark
Reeves, Matthew; University College London
HCMV; cytomegalovirus; CD34+; MCL-1; ELK1
<p>Viral binding and entry provides the first trigger of a cell death response and thus how human cytomegalovirus (HCMV) evades this – particularly during latent infection where a very limited pattern of gene expression is observed – is less well understood. It has been demonstrated that the activation of cellular signalling pathways upon virus binding promotes the survival of latently infected cells by the activation of cell encoded anti-apoptotic responses. In CD34+ cells, a major site of HCMV latency, ERK signalling is important for survival and we now show that the activation of this pathway impacts on multiple aspects of cell death pathways. The data illustrate that HCMV infection triggers activation of pro-apoptotic Bak which is then countered through multiple ERK-dependent functions. Specifically, ERK promotes ELK1 mediated transcription of the key survival molecule MCL-1, along with a concomitant decrease of the pro-apoptotic BIM and PUMA proteins. Finally, we show that the elimination of ELK-1 from CD34+ cells results in elevated Bak activation in response to viral infection, resulting in cell death. Taken together, these data begin to shed light on the poly-functional response elicited by HCMV via ERK-MAPK to promote cell survival.</p>
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UK Medical Research Council
2017-04-26 11:00:58
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/207
Journal of Molecular Biochemistry; Vol 6, No 1 (2017)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/210
2017-04-26T11:00:58Z
JmolBiochem:ED
"170422 2017 eng "
dc
Viral regulation of cell death
Reeves, Matthew; University College London
CD34; latency; cytomegalovirus; HCMV
Lorem Ipsum Press
2017-04-26 11:00:58
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/210
Journal of Molecular Biochemistry; Vol 6, No 1 (2017)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/212
2017-12-08T10:34:20Z
JmolBiochem:REV
"171210 2017 eng "
dc
HCV genetics and genotypes dictate future antiviral strategies
Papageorgiou, Louis; Department of Informatics and Telecommunications, National and Kapodistrian University of Athens, University Campus, Athens 15784, Greece.
Vlachakis, Chrisanthy; Clinical Dietitian-Nutritionist, PhD, Harokopio University, Kallithea, Attica, Greece.
Dragoumani, Konstantina; Clinical Dietitian-Nutritionist, PhD, Harokopio University, Kallithea, Attica, Greece.
Raftopoulou, Sofia; Sotiria Chest Diseases Hospital,152, Mesogion Av., Athens 11527, Greece.
Brouzas, Dimitrios; 1st Department of Ophthalmology, University of Athens, 154, Mesogion Street, Athens 11527, Greece.
Nicolaides, Nicolas C; Division of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children’s Hospital, Athens, Greece.
Chrousos, George P; Division of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children’s Hospital, Athens, Greece.
Saudi Diabetes Study Research Group, King Fahd Center for Medical Research, King Abdulaziz University, Jeddah, Saudi Arabia.
Charmandari, Evangelia; Division of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children’s Hospital, Athens, Greece.
Megalooikonomou, Vasileios; Computer Engineering and Informatics Department, School of Engineering, University of Patras, Patras 26500, Greece.
Vlachakis, Dimitrios; Division of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Computer Engineering and Informatics Department, School of Engineering, University of Patras, Patras 26500, Greece.
Laboratory of Genetics, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos, 11855, Athens, Greece.
HCV; Hepatitis C virus; hepatitis; Genetics; antiviral
<p class="RSCB01ARTAbstract">At the end of the 1980s, the hepatitis C virus (HCV) was cloned and formally identified as the cause of the majority of non-A and non-B hepatitis cases. Today, around 170 million people worldwide are infected with HCV, making it five times more common than infection with the human immunodeficiency virus (HIV). Several methods exist which mediate the spread of infection. One of the most common and efficient is sharing or re-using injecting equipment; studies have indicated that 80-90% of individuals in some populations of intravenous drug users test positive in serum HCV assays. Contracting HCV from infected blood transfusions was also a major cause of infection before screening tests were introduced in the early 1990s. Other possible, but less common, methods of infection transmission include mother-to-child during pregnancy, sexual contact and nosocomial acquisition (for example between surgical or dialysis patients). It appears that concurrent HIV-1 infection increases the risk of HCV transmission via the mother-to-child or sexual routes.</p>
Lorem Ipsum Press
2017-12-08 10:34:20
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/212
Journal of Molecular Biochemistry; Vol 6, No 2 (2017)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/213
2017-12-08T10:34:20Z
JmolBiochem:COMM
"171210 2017 eng "
dc
Insights into the molecular mechanisms of stress and inflammation in ageing and frailty of the elderly
Vlachakis, Dimitrios; Computer Engineering and Informatics Department, School of Engineering, University of Patras, Patras 26500, Greece.
Division of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Laboratory of Genetics, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos, 11855, Athens, Greece.
Zacharaki, Evangelia I; Computer Engineering and Informatics Department, School of Engineering, University of Patras, Patras 26500, Greece.
Tsiamaki, Eirini; Department of Neurology, University of Patras, Patras, Greece.
Koulouri, Maria; Division of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Raftopoulou, Sofia; Division of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
Papageorgiou, Louis; Department of Informatics and Telecommunications, National and Kapodistrian University of Athens, University Campus, Athens 15784, Greece.
Division of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Chrousos, George P; Division of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children’s Hospital, Athens, Greece.
Saudi Diabetes Study Research Group, King Fahd Center for Medical Research, King Abdulaziz University, Jeddah, Saudi Arabia.
Ellul, John; Department of Neurology, University of Patras, Patras, Greece.
Megalooikonomou, Vasileios; Computer Engineering and Informatics Department, School of Engineering, University of Patras, Patras 26500, Greece.
stress; inflammation; frailty; ageing
<p>Frailty is a natural state of physical, cognitive and mental decline that is expected in the elderly. The role of inflammation in the pathogenesis of frailty has been hypothesized, and so far many studies have been performed in order to understand the mechanism of action underlying this association. Recent studies support this hypothesis and show a clear association between inflammation, frailty, and age-related disease. Chronic inflammation is key pathophysiologic process that contributes to the frailty directly and indirectly through other intermediate physiologic systems, such as the musculoskeletal, endocrine, and hematologic systems. The complex multifactorial etiologies of frailty also include obesity and other age-related specific diseases. Herein, we investigate the link between chronic inflammation and frailty of the older people. In particular, we present an up-to-date review of the role of cytokines, interleukins, cardiovascular abnormalities, chronic high blood pressure, hyperlipidemia and diabetes in relation to the severity of frailty in the elderly.</p>
Lorem Ipsum Press
2017-12-08 10:34:20
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/213
Journal of Molecular Biochemistry; Vol 6, No 2 (2017)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/214
2017-12-08T10:34:20Z
JmolBiochem:ART
"171210 2017 eng "
dc
Retroviral proteases: correlating substrate recognition with both selected and native inhibitor resistance
Laco, Gary S; Laco Science Institute
active-site inhibitor; cross-species transmission; native resistance; retroviral protease; selected resistance; substrate-grooves; anti-parallel beta-sheet
<p class="WWDefault">A diverse group of retroviral proteases were analyzed to correlate mechanisms of substrate recognition with resistance to HIV-1 protease active-site inhibitors. Here it was shown that HIV-1 protease utilized a pathway common to many retroviral proteases, for recognition of mutated Gag/Pol cleavage sites, in order to become resistant to active-site inhibitors. While HIV-1 and HIV-2 resulted from independent cross-species transmissions of simian immunodeficiency virus into humans, HIV-2 has native primary resistance to many HIV-1 protease inhibitors as do many other retroviral proteases. The native multi-drug resistance of those proteases contributed to the lack of treatments for the respective life-long infections. Analysis of interactions between retroviral proteases and Gag/Pol substrates revealed that protease interactions weighted towards cleavage site residues P4-P4' resulted in inhibitor sensitivity, while interactions weighted towards residues P12-P5/P5'-P12' gave inhibitor resistance. In addition, a mechanism was identified for human T-cell leukemia virus type-1 protease that allowed re-weighting of the protease interactions with substrate residues P4-P4' and P12-P5/P5'-P12' using anti-parallel beta-sheets that connected the protease flaps to the substrate-grooves. Those anti-parallel beta-sheets are common to all studied retroviral proteases. The critical role of the retroviral protease substrate-grooves in substrate recognition and inhibitor resistance makes them a potential target.</p>
Lorem Ipsum Press
2017-12-08 10:34:20
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/214
Journal of Molecular Biochemistry; Vol 6, No 2 (2017)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/206
2017-12-08T10:34:20Z
JmolBiochem:REV
"171210 2017 eng "
dc
Zika virus infection: a review of available techniques towards early detection
Glover, Kathleen KM; University of Manitoba
Coombs, Kevin; University of Manitoba
zika virus; zika virus diagnosis; pandemic
<p>Zika virus belongs to the family Flaviviridae as do other viruses like Dengue, West Nile and Yellow Fever. They are arboviruses transmitted by the Aedes species of mosquito. Zika virus was first isolated in rhesus monkeys in Uganda in 1947. Human infections of the virus were found between the 1960s and 1980s in Africa, the Americas, Asia and the Pacific. The similarity in clinical presentation in Zika-infected patients compared with Dengue caused infections to be previously misdiagnosed as Dengue infection. The Zika virus pandemic in 2015 created a lot of concern globally because of little information about available techniques, samples as well as no available antiviral and vaccines for treatment and vaccination against infection. In addition, the vectors identified for transmission, Aedes aegypti and Aedes albopictus, were of great concern due to their ability to survive both temperate and tropical climatic conditions, hence indicating the possible global spread of Zika virus infection. Almost two years after the report of infection in pregnant women in Brazil resulting in microcephalic babies, Zika virus was identified as a public health problem. Thus, a lot of research into early detection and prevention has been conducted to control the spread of the virus. This review paper highlights available information on techniques currently available for diagnosis of infection caused by Zika virus.</p> <p> </p> <p> </p>
Lorem Ipsum Press
2017-12-08 10:34:20
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/206
Journal of Molecular Biochemistry; Vol 6, No 2 (2017)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/217
2018-06-04T22:52:42Z
JmolBiochem:ART
"180601 2018 eng "
dc
Potential interference of aluminum chlorohydrate with estrogen receptor signaling in breast cancer cells
Gorgogietas, Vyron A
Tsialtas, Ioannis
Sotiriou, Natalie
Laschou, Vasiliki C
Karra, Aikaterini G
Leonidas, Demetres D
Chrousos, George P
Protopapa, Evagelia
Psarra, Anna - Maria G; University of Thessaly
Department of Biochemistry and Biotechnology
Biopolis
41500 Larissa
Greece
Estrogen receptor; aluminum; breast cancer; mitochondria; metalloestrogen
<p>Aluminum salts are widely used as the active antiperspirant in underarm cosmetic. Experimental observations indicate that its long term application may correlate with breast cancer development and progression. This action is proposed to be attributed, among others, to aluminum possible estrogen-like activities. In this study we showed that aluminum, in the form of aluminum chlorohydrate (ACH), caused increase in estrogen receptor alpha (ERα) protein levels, in ERα-positive MCF-7 cells. This effect was accompanied by moderate activation of Estrogen Response Elements (ERE)-driven reporter gene expression and 20%-50% increase in certain estrogen responsive, ERE-independent genes expression. Genes affected were ERα, p53, cyclin D1, and c-fos, crucial regulators of breast cancer development and progression. ACH-induced genes expression was eliminated in the presence of the estrogen antagonist: ICI 182780, in MCF-7 cells, whereas it was not observed in ERα-negative MDA-MB-231 breast cancer cells, indicating aluminum interference with estrogen signaling. Moreover, ACH caused increase in the perinuclear localization of estrogen receptor alpha in MCF-7 breast cancer cells and increase in the mitochondrial Bcl-2 protein, possibly affecting receptors-mediated mitochondrial actions and mitochondrial-dependent apoptosis. ACH-induced perinuclear localization of estrogen receptor beta was also observed in MDA-MB-231. Our findings indicate that aluminum actions on estrogen receptors protein level and subcellular localization possibly affect receptors-mediated actions and thus, aluminum interference with estrogen signaling.</p>
Lorem Ipsum Press
Bodossaki Foundation; Operational Program “Education and Lifelong Learning” of NSRF- Research Funding Program: “Archimedes III”; Masters University
2018-06-04 22:46:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/217
Journal of Molecular Biochemistry; Vol 7, No 1 (2018)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/218
2018-06-04T22:46:00Z
JmolBiochem:ART
"180601 2018 eng "
dc
Mannose-rich guar gum nanoparticles as a novel therapeutic drug against inflammatory diseases
Ghosh, Nandita
Mitra, Shinjini
Biswas, Silpak
Ray Banerjee, Ena
<p>The potential to deliver nanoparticles, like polymer-based nanoparticles that can be enriched with functional groups to ensure entry into cells, directly into targeted cells is important for the therapy of inflammatory diseases. Plant-derived nanoparticles, with inherent anti-inflammatory activity and modified to allow receptor-mediated uptake, can be used as effective therapy with minimal side effects. The particle used in this study is an edible polysaccharide, derived from Cyamopsis tetragonoloba, with a galactomannan component. The particle was made mannose-rich to increase specificity towards cells expressing mannose receptors, and initially tagged with rhodamine isothiocyanate to trace its path. This study aimed to determine the therapeutic effect of the guar gum nanoparticle (GN) in vitro and in vivo in inflammatory diseases. In vitro studies on RAW 264.7 cells showed successful uptake of the nanoparticle, in a short duration of time, via their mannose receptors. Nitric oxide and MTS assays showed anti-inflammatory effects of GN. In vivo mouse model of thioglycollate-induced peritonitis showed significant decrease in inflammation, indicating its anti-inflammatory effect, and increase in clonogenic potential, indicating its regenerative potential, on intraperitoneal administration of GN. The results reflect the potential of the nanoparticle in cellular trafficking, site- specific drug delivery and bioimaging applications.</p>
Lorem Ipsum Press
2018-06-04 22:46:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/218
Journal of Molecular Biochemistry; Vol 7, No 1 (2018)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/219
2018-06-04T22:46:00Z
JmolBiochem:ART
"180601 2018 eng "
dc
Galectin-12 colocalizes with splicing factor-rich speckles and shuttles between the nucleus and cytoplasm in colon cancer cells
Katzenmaier, Eva; Department of Applied Tumor Biology, Institute of Pathology, University Hospital Heidelberg, Germany
Clinical Cooperation Unit Applied Tumor Biology, DKFZ (German Cancer Research Center), Heidelberg, Germany
Stark, Hans-Jürgen; Department of Applied Tumor Biology, Institute of Pathology, University Hospital Heidelberg, Germany
Clinical Cooperation Unit Applied Tumor Biology, DKFZ (German Cancer Research Center), Heidelberg, Germany
Gebert, Johannes; Department of Applied Tumor Biology, Institute of Pathology, University Hospital Heidelberg, Germany
Clinical Cooperation Unit Applied Tumor Biology, DKFZ (German Cancer Research Center), Heidelberg, Germany
Kopitz, Jürgen; Department of Applied Tumor Biology, Institute of Pathology, University Hospital Heidelberg, Germany
Clinical Cooperation Unit Applied Tumor Biology, DKFZ (German Cancer Research Center), Heidelberg, Germany
colorectal cancer; lectins; galectins; nucleocytoplasmic shuttling; Tet-On system; Tet-regulated gene expression
<p>Several members of the glycan-binding family of galectins have been linked to colon cancer initiation and progression while the role of galectin-12 in this malignancy is largely unexplored. In previous studies we observed expression of galectin-12 in normal colon epithelium in contrast to its lack of expression in colorectal cancer tissues. In order to gain insight into its molecular function we established a genetically engineered colon cancer model cell line, which facilitates inducible and reconstituted expression of LGALS12 transgene at physiological levels in an isogenic background. Regulation of transgene expression by doxycycline was confirmed at the transcript- and protein level in a time- and dose-dependent manner for two independent clones. Reconstituted galectin-12 expression did not affect cell morphology and proliferation. Analysis of its subcellular distribution showed that galectin-12 resides in and shuttles between the nucleus and cytoplasm. More detailed analysis by double-immunofluorescence and confocal microscopy revealed colocalization of galectin-12 with splicing-factor rich nuclear speckles, hinting towards a potential role of galectin-12 in pre-mRNA splicing and processing. Therefore, the established model cell line represents a powerful tool to investigate the functional impact of galectin-12 reconstitution on colon cancer tumorigenesis.</p>
Lorem Ipsum Press
Stiftung fuer Krebs- und Scharlachforschung
2018-06-04 22:46:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/219
Journal of Molecular Biochemistry; Vol 7, No 1 (2018)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/219/759
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/219/791
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/221
2018-06-04T22:46:00Z
JmolBiochem:ART
"180601 2018 eng "
dc
Selective lactase deficiency is common in pediatric patients undergoing upper endoscopy
Goodwin, Annie; Texas Children's Hospital, Baylor College of Medicine, USA
Karam, Lina B; Texas Children's Hospital, Baylor College of Medicine, USA
Gopalakrishna, G S
Kellermayer, Richard; Texas Children's Hospital, Baylor College of Medicine, USA
lactose intolerance: lactase deficiency; children
<p>Lactase deficiency can lead to significant symptoms in the pediatric population. To date, few studies have examined the prevalence of enzyme testing-based lactase and other disaccharidase deficiencies (DDs) in pediatric patients undergoing upper endoscopic evaluation. The primary objective of this study was to determine the prevalence of selective lactase and other DDs amongst a large cohort of pediatric patients with and without inflammatory bowel disease (IBD: Crohn’s disease and ulcerative colitis) via a chart review of 739 patients who underwent esophago-gastro-dudenoscopy EGD between April 2010 and August 2016. We identified 560 pediatric patients (ages 1-18 years) who underwent mucosal enzyme testing at the time of their EGD. The overall rate of lactase deficiency (LD) was 39%. LD positively correlated with age (p=0.00017), but there was no significant difference between age matched IBD and non-IBD patients (45% vs. 42% p=0.68). Four patients (0.17%) were found to have selective maltase deficiency. No selective sucrase or palatinase deficiency was identified. Statistically significant differences occurred in lactase deficiency amongst patients of different races. In conclusion, lactase deficiency is a relatively common finding in children undergoing EGD though at no increased rate amongst the IBD patient population. Disaccharidase testing should be considered in pediatric patients undergoing EGD.</p>
Lorem Ipsum Press
2018-06-04 22:46:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/221
Journal of Molecular Biochemistry; Vol 7, No 1 (2018)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/222
2018-06-04T22:46:00Z
JmolBiochem:ART
"180601 2018 eng "
dc
Adenylyl cyclases and TRPV4 mediate Ca2+/ cAMP dynamics to enhance fluid flow-induced osteogenesis in osteocytes
Moore, Emily; Columbia University in the City of New York
Ryu, Han Seul
Zhu, Ya Xing
Jacobs, Christopher R
Primary cilium, osteocyte, AC6, AC3, TRPV4, mechanotransduction, calcium, cAMP
<p>Bone adapts to physical forces and this process is dependent on osteocyte mechanotransduction. One way osteocytes sense mechanical stimulation is through the primary cilium, a sensory organelle that triggers intracellular signaling cascades in response to fluid shear. Our lab previously determined that flow-induced ciliary Ca<sup>2+</sup> influx and changes in cytosolic cAMP levels are critical for osteogenesis. We also identified two proteins important for osteocyte mechanotransduction: transient receptor potential vanilloid 4 (TRPV4) and adenylyl cyclase 6 (AC6). Interestingly, disrupting the Ca<sup>2+</sup>-binding ability of these proteins results in loss of function. Although knockdowns of TRPV4 and AC6 disrupt osteogenesis, there is no definitive evidence linking them to Ca<sup>2+</sup>/ cAMP dynamics that facilitate osteocyte mechanotransduction. We therefore transfected MLO-Y4 osteocytes with AC3/6 and TRPV4 overexpression plasmids that fail to interact with Ca<sup>2+</sup> and observed the response to fluid shear. Indeed, mutant groups exhibited adverse changes in cAMP and lower mRNA expression of an osteogenic marker, COX-2, at the onset of flow. This pattern persisted for AC3 and TRPV4 but we detected no difference in AC6 at longer exposure to flow. These results suggest TRPV4 and ACs mediate Ca<sup>2+</sup>/ cAMP dynamics that are important for osteocyte mechanotransduction. These mechanisms are potential targets for therapeutics to combat bone loss and should be investigated further.</p>
Lorem Ipsum Press
2018-06-04 22:46:00
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/222
Journal of Molecular Biochemistry; Vol 7, No 1 (2018)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/236
2018-10-02T17:07:03Z
JmolBiochem:ART
"181003 2018 eng "
dc
Overexpression of human DKK1 via rAAV vector-mediated gene transfer stimulates chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells
Venkatesan, Jagadeesh Kumar; Center of Experimental Orthopaedics, Saarland University Medical Center and Saarland University, Homburg/Saar, Germany
Schmitt, Gertrud; Center of Experimental Orthopaedics, Saarland University Medical Center and Saarland University, Homburg/Saar, Germany
Speicher-Mentges, Susanne; Center of Experimental Orthopaedics, Saarland University Medical Center and Saarland University, Homburg/Saar, Germany
Madry, Henning; 1Center of Experimental Orthopaedics, Saarland University Medical Center and Saarland University, Homburg/Saar, Germany
2Department of Orthopaedic Surgery, Saarland University Medical Center and Saarland University, Homburg/Saar, Germany
Cucchiarini, Magali; Center of Experimental Orthopaedics, Saarland University Medical Center and Saarland University, Homburg/Saar, Germany
cartilage repair; MSCs; rAAV; DKK1; chondrogenic differentiation
<p class="Default">Enhancing the chondroregenerative activities of mesenchymal stem cells via therapeutic gene transfer as reinforced sources of implantable cells in sites of cartilage injury is a promising tool to improve the natural processes of cartilage repair. In the present study, we show that overexpression of the Dickkopf-related protein 1 (DKK1) via clinically adapted recombinant adeno-associated viral (rAAV) vectors is capable of significantly stimulating proliferative, anabolic, and chondrodifferentiation events in primary human mesenchymal stem cells compared with control (reporter rAAV lacZ) transduction over an extended period of time in vitro (21 days). Strikingly, administration of the rAAV DKK1 candidate vector concomitantly restrained unwanted osteogenic and hypertrophic differentiation outcomes in these cells. These findings reveal the possible future benefits of such an approach to treat articular cartilage lesions in relevant experimental models in vivo.</p>
Lorem Ipsum Press
This work was funded by the German Osteoarthritis Foundation (Deutsche Arthrose-Hilfe e.V.)
2018-10-02 17:07:03
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/236
Journal of Molecular Biochemistry; Vol 7, No 2 (2018)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/242
2018-10-02T17:07:03Z
JmolBiochem:ART
"181003 2018 eng "
dc
Impact of a stress management program on weight loss, mental health and lifestyle in adults with obesity: a randomized controlled trial
Xenaki, Niovi
Bacopoulou, Flora; Flora Bacopoulou, MD, PhD, Assistant Professor of Pediatrics-Adolescent Medicine
Tel: +306973208208, +306955680000, Fax: +30 2132013237, Email: bacopouf@hotmail.com, fbacopoulou@med.uoa.gr
Kokkinos, Alexandros
Nicolaides, Nicolas C; Medical School Athens
Chrousos, George P
Darviri, Christina
obesity; stress management program; weight loss; Greek adults; stress; depression; anxiety; lifestyle; health locus of control
<p class="Standard">Aim: To evaluate the impact of a stress management program on weight loss, depression, anxiety and stress as well as on the adoption of healthy lifestyle in adults with obesity. Methods: Adults with obesity who sought help for weight loss at a medical obesity clinic were consecutively enrolled in the study and were randomly assigned to the intervention or control group. All participants received standard instructions for a healthy lifestyle. The intervention group attended an 8-week stress management program that comprised diaphragmatic breathing, progressive muscle relaxation, guided visualization and instructions about healthy nutrition/dietary habits. Anthropometric parameters were assessed and several questionnaires were completed by all participants, at the beginning and at the end of the study. Results: A total of 45 adults (mean age±SD 45.7±10.55 years) with obesity were enrolled in the study; 22 in the intervention group and 23 in the control group. Participants in the two groups were matched for age and BMI. Participants in the intervention group achieved a significantly larger reduction in BMI compared to the control group (ΔBMI -3.1 vs. -1.74 kg/m<sup>2 </sup>respectively, <em>P</em><0.001). In addition, they displayed ameliorated depression and anxiety scores and a reduction in the health locus of control based on chance.</p>
Lorem Ipsum Press
2018-10-02 17:07:03
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/242
Journal of Molecular Biochemistry; Vol 7, No 2 (2018)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/240
2018-10-02T17:07:03Z
JmolBiochem:ART
"181003 2018 eng "
dc
Introducing Thetis: a comprehensive suite for event detection in molecular dynamics
Picasi, Eleni
Tartas, Athanasios
Megalooikonomou, Vasileios
Vlachakis, Dimitrios
molecular dynamics,helical structure, conformational analysis, statistical analysis
<p>A suite of computer programs has been developed under the general name Thetis, for monitoring structural changes during molecular dynamics (MD) simulations on proteins. Conformational analysis includes estimation of structural similarities during the simulation and analysis of the secondary structure with emphasis on helices. In contrast to available freeware dealing with MD snapshots, Thetis can be used on a series of consecutive MD structures, thus allowing a detailed conformational analysis over the time course of the simulation.</p>
Lorem Ipsum Press
2018-10-02 17:07:03
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/240
Journal of Molecular Biochemistry; Vol 7, No 2 (2018)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/247
2019-07-25T22:57:18Z
JmolBiochem:ART
"190728 2019 eng "
dc
Greek Validation of Emotional Eating Scale for Children and Adolescents
Kalogiratou, Despina S
Bacopoulou, Flora
Kanaka-Gantenbein, Christina
Vlachakis, Dimitrios
Gerakini, Orsalia
Chrousos, George P
Darviri, Christina
emotional eating scale; EES-C;CDI; STAIC
<p><strong>Aim:</strong> In this study, we focused on the Greek validation of the Emotional Eating Scale for Children and Adolescents (EES-C). <strong>Methods:</strong> Our sample consisted of 150 students in primary and secondary school settings from two different areas of Greece. Child Depression Inventory (CDI) and State and Trait Anxiety in Children (STAIC) were also used for validation purposes. <strong>Results:</strong> The principal component factor analysis for construct validity generated three subscales: eating in response to anger/anxiety, feeling unsettled and depression. The EES-C tool was found with high internal reliability (Cronbach's Alpha 0.917). <strong>Conclusions:</strong> EES-C is a valid and reliable instrument to detect the emotional eating in children and adolescents in Greece.</p>
Lorem Ipsum Press
2019-07-25 22:57:18
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/247
Journal of Molecular Biochemistry; Vol 8, No 1 (2019)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/243
2019-07-25T22:57:18Z
JmolBiochem:ART
"190728 2019 eng "
dc
Stress Management in Women with Hashimoto’s thyroiditis: A Randomized Controlled Trial
Markomanolaki, Zoe S; Postgraduate Course of Stress Management and Health Promotion, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece
Tigani, Xanthi; Postgraduate Course of Stress Management and Health Promotion, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece
Siamatras, Thomas; Naval Hospital of Athens, Greece
Bacopoulou, Flora; Center for Adolescent Medicine and UNESCO Chair on Adolescent Health Care, First Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, Aghia Sophia Children’s Hospital, Athens 11527, Greece
Tsartsalis, Athanasios; Naval Hospital of Athens, Greece
Artemiadis, Artemios; Postgraduate Course of Stress Management and Health Promotion, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece
Megalooikonomou, Vasileios; Computer Engineering and Informatics Department, School of Engineering, University of Patras, Patras 26500, Greece
Vlachakis, Dimitrios P; Laboratory of Genetics, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos, 11855, Athens, Greece
Chrousos, George P; Center for Adolescent Medicine and UNESCO Chair on Adolescent Health Care, First Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, Aghia Sophia Children’s Hospital, Athens 11527, Greece
Darviri, Christina; Postgraduate Course of Stress Management and Health Promotion, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece
Hashimoto's thyroiditis; autoimmune thyroiditis; stress management; intervention; anti-thyroid antibodies; anti-TG; stress; anxiety; depression; lifestyle
<p><strong>Aim:</strong> Stress has been implicated in the pathogenesis of Hashimoto's thyroiditis (HT), nevertheless evidence is scarce regarding the effect of stress management on individuals suffering from HT. The purpose of this study was to evaluate the impact of an 8-week stress management intervention on the anti-thyroid peroxidase (anti-TPO) and anti-thyroglobulin (anti-TG) antibodies as well as thyroid-stimulating hormone (TSH) levels of women with HT. Secondary endpoints included the effect on the patients’ lifestyle, body mass index (BMI), depression, anxiety and stress. <strong>Methods:</strong> This was a two-arm parallel group (stress management intervention vs. standard care groups) randomized controlled study. Adult women with Hashimoto's thyroiditis, completed questionnaires on stress, anxiety, depression and lifestyle, at the beginning of the programme and 8 weeks later. Laboratory thyroid function tests (anti-TPO, anti-TG antibodies and TSH) were also measured at baseline and at the end of the study. <strong>Results:</strong> A total of 60 women with HT, aged 25-76 years, participated in the study (30 patients in each group). After eight weeks, patients in the intervention group demonstrated statistically significant beneficial decrements in the rate change of anti-TG titers and the levels of stress, depression and anxiety as well as better lifestyle scores, compared to the control group.</p> <p> </p><p> </p><p> </p>
Lorem Ipsum Press
2019-07-25 22:57:18
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/243
Journal of Molecular Biochemistry; Vol 8, No 1 (2019)
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Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/244
2019-07-25T23:31:57Z
JmolBiochem:LETT
"190728 2019 eng "
dc
An integrated pipeline for the pest management of Bactrocera oleae
Konstantopoulos, Dimitris
Cosmidis, Nikos; Agricultural University of Athens
Bactrocera oleae; incectiside; Candidatus Erwinia dacicola; Insect population control
Lorem Ipsum Press
Department of genetics, Abricultural university of Athens
2019-07-25 22:57:18
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/244
Journal of Molecular Biochemistry; Vol 8, No 1 (2019)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/245
2019-07-25T22:57:18Z
JmolBiochem:ED
"190728 2019 eng "
dc
State of the art immunogenetics and immunoinformatics
Papakonstantinou, Eleni; Laboratory of Genetics, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, Athens, Greece
Vlachakis, Dimitrios; Laboratory of Genetics, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, Athens, Greece
immunogenetics; immunoinformatics
Lorem Ipsum Press
2019-07-25 22:57:18
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/245
Journal of Molecular Biochemistry; Vol 8, No 1 (2019)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/246
2019-07-25T22:57:18Z
JmolBiochem:ART
"190728 2019 eng "
dc
Antitumor activity of Neem leaf Extract and Nimbolide on Ehrlich Ascites Carcinoma Cells in Mice
Mohamed, Faten Zahran
Basuni, Mohamed Abd El-Hakim
Haikel, Noha Gamal
nimbolide; Neem leaf; antioxidant; Ehrlich ascites carcinoma cells
<p><strong>Background:</strong> Azadirachta indica (Neem) has been used traditionally for many centuries. Some impressive therapeutic qualities have been discovered. Aim: Our study aims to investigate the in vivo antitumor and antioxidant activities of Ethanolic Neem Leaf Extract (ENLE) and its fraction called Nimbolide, a limonoid present in leaves and flowers of the neem tree (Azadirachta indica). Also, to study the side effects of the ethanolic Neem leaf extract and Nimbolide fraction on the liver and kidney. <strong>Materials & Methods:</strong> We assessed the effect of nimbolide and ethanolic Neem leaves extract (ENLE) on replicative lifespan prolongation in vitro and on the levels of malondialdehyde (MDA), nitric oxide (NO), catalase, glutathione peroxidase (GPx), Caspase-3, and Cytochrome c. Also, our study estimated their effect on Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Total Protein (TP), Albumin (Alb), bilirubin, urea and creatinine. <strong>Results:</strong> Ethanolic neem leaves extract and nimbolide resulted in increases in replicative lifespan. Also, they showed a significant decrease in malondialdehyde and nitric oxide and an increase in catalase, glutathione peroxidase, caspase-3 activities, and cytochrome c concentration. Hence, it may be possible that Nimbolide and ENLE decrease lipid peroxidation level due to their antioxidant effect and enhance apoptosis. The Neem leaves extract and nimbolide showed no side effects on liver and kidney. Also, they showed a significant protection for both liver and kidney histopathologically.</p>
Lorem Ipsum Press
2019-07-25 22:57:18
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/246
Journal of Molecular Biochemistry; Vol 8, No 1 (2019)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/250
2021-01-07T15:50:51Z
JmolBiochem:ART
"201230 2020 eng "
dc
Reliability and Validity of the Newly Diagnosed Breast Cancer Stress Scale in the Greek Population
Charalampopoulou, Maria
Syrigos, Konstantinos
Filopoulos, Evaggelos
Megalooikonomou, Vasileios
Vlachakis, Dimitrios
Chrousos, George
Darviri, Christina
Validation, psychometric instrument, newly diagnosed, breast cancer, distress
<p><strong> </strong></p> <p>Objective: To examine the validity and the reliability of a novel measurement tool, the Newly Diagnosed Breast Cancer Stress Scale (NDBCSS) in the Greek population. The tool aimed to assess distress in patients recently diagnosed with breast cancer. Methods: We performed a principal component analysis (PCA) of the 17 items of the scale. Results: The PCA resulted in 4 factors: 1. Personal life, 2. Procedural issues, 3. Facing challenges and 4. Psychological load. All subscales showed satisfactory internal consistency and variance, relative to theoretical score ranges. Subscale scores and total score were significantly correlated with perceived stress and hospital anxiety and depression scale, implying good criterion validity. Associations with social, demographic and disease related information were also found. Conclusions: The NDBCSS resulted in acceptable reliability and good validity, and was considered as a valuable tool for health-care workers and oncologists to measure psychological distress in early stage of breast cancer.</p> <p><strong> </strong></p>
Lorem Ipsum Press
2021-01-07 15:50:51
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/250
Journal of Molecular Biochemistry; Vol 9, No 1 (2020)
en
http://www.jmolbiochem.com/index.php/JmolBiochem/article/download/250/899
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/251
2021-01-07T15:50:51Z
JmolBiochem:ART
"201230 2020 eng "
dc
Effects of the Pythagorean Self Awareness Intervention on Childhood Emotional Eating and Psychological Wellbeing: a Pragmatic Trial
Kalogiratou, Despina S.; Postgraduate Course Stress Management and Health Promotion, Medical School, National and Kapodistrian University of Athens, 4 Soranou Ephessiou Str., Athens 11527, Greece
Bacopoulou, Flora; First Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, Aghia Sophia Children’s Hospital, National and Kapodistrian University of Athens, 1 Thivon Str., Athens 11527, Greece
Kanaka-Gantenbein, Christina; Postgraduate Course Stress Management and Health Promotion, Medical School, National and Kapodistrian University of Athens, 4 Soranou Ephessiou Str., Athens 11527, Greece
First Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, Aghia Sophia Children’s Hospital, National and Kapodistrian University of Athens, 1 Thivon Str., Athens 11527, Greece
Tigani, Xanthi; Postgraduate Course Stress Management and Health Promotion, Medical School, National and Kapodistrian University of Athens, 4 Soranou Ephessiou Str., Athens 11527, Greece
Gerakini, Orsalia; Postgraduate Course Stress Management and Health Promotion, Medical School, National and Kapodistrian University of Athens, 4 Soranou Ephessiou Str., Athens 11527, Greece
Vlachakis, Dimitrios
Chrousos, George P.; Postgraduate Course Stress Management and Health Promotion, Medical School, National and Kapodistrian University of Athens, 4 Soranou Ephessiou Str., Athens 11527, Greece
First Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, Aghia Sophia Children’s Hospital, National and Kapodistrian University of Athens, 1 Thivon Str., Athens 11527, Greece
Darviri, Christina; Postgraduate Course Stress Management and Health Promotion, Medical School, National and Kapodistrian University of Athens, 4 Soranou Ephessiou Str., Athens 11527, Greece
stress;- emotional eating;- primary school;- stress management;-Pythagorean Self Awareness
<p>Background: Emotional eating is the tendency to regulate negative emotions (such as stress, depression and anxiety) through increased or unhealthy food intake. Emotion management, such as stress management could be an appropriate approach to prevent or control maladaptive eating behavior among children. Materials and methods: This is a controlled pragmatic trial, testing the effects of Pythagorean Self Awareness Intervention (PSAI), a cognitive stress management program in a primary school setting. The eligibility criterion was being a 4<sup>th</sup> grade active pupil. Participants were randomly assigned to either the intervention group (n = 23) or the control group (n = 22). Self-report measures were used for the evaluation of various variables at the beginning and the end of the 8-week monitoring period. Descriptive and inferential statistic methods were used for the statistical analysis. Results: At the end of the 8-week period pupils in the intervention group experienced statistically significant reduction in stress (SIC, p < 0.001), anxiety (STAIC-trait anxiety, p = 0.019, STAIC-state anxiety, p = 0.006), guilt (p < 0.001) and emotional eating (EES-C, p < 0.001) and an increase in Mediterranean diet quality (KIDMED, p = 0.001) and pride (p < 0.001). No statistically significant differences between the two groups were recorded regarding depression symptoms (CDI, p = 0.551) and shame (p = 0.120). Conclusions: PSAI had positive effects on a sample of primary school attendants’ emotional eating and psychological state.</p>
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2021-01-07 15:50:51
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/251
Journal of Molecular Biochemistry; Vol 9, No 1 (2020)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/253
2021-01-07T15:50:51Z
JmolBiochem:ART
"201230 2020 eng "
dc
An 8-week Stress Management Program in Information Technology Professionals and the Role of a New Cognitive Behavioral Method: a Pilot Randomized Controlled Trial
Sioula, Evangelia K; National and Kapodistrian University of Athens, Postgraduate Course Stress Management and Health Promotion, School of Medicine
Tigani, Xanthi
Artemiadis, Artemios
Vlachakis, Dimitrios
Chrousos, George P
Darviri, Christina
Alexopoulos, Evangelos C
Health promotion, Occupational stress, Relaxation, Stress management
<p>Aim: The aim of this study is to compare and evaluate the sort-term benefits of the effects of an 8-week stress management techniques in information technology professionals. Methods: In this parallel randomized controlled trial, participants were randomly assigned to either the stress management group (n=40; relaxation breathing, progressive muscle relaxation, guided imagery) or in the Pythagorean Self awareness group (n=41). Self-reported validated measures were used to evaluate perceived stress, health locus of control, anxiety and depression. Results: All groups were found with significantly better cognitive speed and verbal memory at the end of the follow-up. Taking into account the group by time interaction coefficients, PSAT was found significantly superior to standard SM with regards to depression, emotional intelligence, lifestyle and personal control and verbal memory suggesting that verbal memory improvement through time should be mostly attributed to PSAT. On the other hand, the cognitive speed improvement during follow-up should be attributed to both interventions. Conclusions: These findings provide important insight into the role of stress management. Future studies should focus on randomized, controlled trials with larger samples and longer follow-up times.</p> <p> </p>
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Postgraduate Course Stress Management and Health Promotion, School of Medicine, National and Kapodistrian University of Athens, 4 Soranou Ephessiou Str., 11527, Athens, Greece
2021-01-07 15:50:51
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/253
Journal of Molecular Biochemistry; Vol 9, No 1 (2020)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/254
2021-01-07T15:50:51Z
JmolBiochem:ART
"201230 2020 eng "
dc
Τhe Effect of the Pythagorean Self-Awareness Intervention on Psychological, Lifestyle and Cognitive Measures of a Community Sample
Zigkiri, Eleni; Postgraduate Course ''Stress Science & Health Promotion'', Medical School, National & Kapodistrian University of Athens-Greece
Nicolaides, Nicolas; Postgraduate Course Stress Science and Health Promotion, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece;
Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, Athens 11527, Greece;
Bacopoulou, Flora; Postgraduate Course Stress Science and Health Promotion, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece;
Center for Adolescent Medicine and UNESCO Chair on Adolescent Health Care, First Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, Athens 11527, Greece.
Simos, Dimitrios; Postgraduate Course Stress Science and Health Promotion, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece;
Vlachakis, Dimitrios; Laboratory of Genetics, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, Athens, Greece
Chrousos, George; Postgraduate Course Stress Science and Health Promotion, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece;
Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, Athens 11527, Greece;
Division of Endocrinology and Metabolism, Biomedical Research Foundation of the Academy of Athens, Athens 11527, Greece;
Clinical and Translational Research Endocrine Unit, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece;
Center for Adolescent Medicine and UNESCO Chair on Adolescent Health Care, First Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, “Aghia Sophia” Children’s Hospital, Athens 11527, Greece.
Darviri, Christina; Postgraduate Course Stress Science and Health Promotion, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece;
cognitive-behavioral intervention; community sample; Pythagorean Self-Awareness Intervention; stress; stress management
<p>Background: The aim of this study was to evaluate the effect of a novel cognitive-behavioral stress management intervention, termed “Pythagorean Self-Awareness Intervention” (PSAI). Materials and Methods: The PSAI was applied to a community sample for eight weeks. Measurements included demographic characteristics, daily habits, sleep quality, fatigue, perceived stress levels and depressive symptoms. Cognitive function was measured by the Symbol Digits Modality Test (SDMT), the California Verbal Learning Test (CVLT) and the Brief Visuospatial Memory Test-Revised (BVMT-R). Results: The high-compliance group showed statistically significant differences in healthy lifestyle and personal control choices (HLPCQ; p=0.006), sleep quality (PSQI; p=0.007), self-regulation eating behavior (SREBQ; p=0.009), perceived stress (PSS; p=0.001) and depressive symptoms (p=0.003). Conclusions: Frequent practice of the PSAI had a positive effect on the psychological, lifestyle, and cognitive measures of the community sample. Larger studies are needed to establish the PSAI as an effective stress management method.</p>
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2021-01-07 15:50:51
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/254
Journal of Molecular Biochemistry; Vol 9, No 1 (2020)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/255
2021-01-07T15:50:51Z
JmolBiochem:ART
"201230 2020 eng "
dc
The Effect of Vitamin D Supplementation on Cardiometabolic Risk Factors and Mental Health Symptoms in Obese Children
Giannios, Christos; Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children's Hospital
Gennitsaridi, Sofia; Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children's Hospital
Kolaitis, Gerasimos; Department of Child Psychiatry, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children's Hospital
Nicolaides, Nicolas; Division of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens
Farakla, Ioanna; Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children's Hospital
Karampatsou, Sofia; Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children's Hospital
Papageorgiou, Ifigeneia; Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children's Hospital
Kassari, Penio; Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children's Hospital
Papadopoulou, Penelope; Department of Child Psychiatry, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children's Hospital
Charmandari, Evangelia; Division of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, “Aghia Sophia” Children's Hospital
children; vitamin D; treatment; obesity; metabolic syndrome; mental health
<p>Objective: Decreased 25(OH)-vitamin D concentrations represent a risk factor for the development of cardiometabolic and mental health disorders in adults. We investigated the effect of vitamin D supplementation on cardiometabolic risk factors and mental health symptoms in overweight and obese children, and adolescents with vitamin D deficiency or insufficiency. Patients and Methods: Two hundred and twenty (n=220) overweight and obese children and adolescents [Mean age ± SEM: 10.24±0.17 years; BMI ± SEM: 26.69±0.28 kg/m2; BMI z-score ± SEM: 2.42±0.07; males: 114 (51.8%), females: 106 (48.2%)] with vitamin D deficiency or insufficiency were studied prospectively and randomly assigned into the supplementation (n=109) or the control group (n=111). Participants in the supplementation group received 50,000 IU cholecalciferol once a week for 6 weeks followed by a maintenance dose. Blood samples for determination of 25(OH)-vitamin D, endocrinologic and cardiometabolic parameters were obtained at baseline and 12 months later. Systolic and diastolic blood pressure was determined twice and the mean value was calculated. Mental health was assessed by questionnaires at baseline and 12 months later. Results: Subjects in the supplementation group had significantly lower BMI (p=0.010), hsCRP (p=0.048) and total cholesterol (p=0.015), and higher HDL (p<0.001) concentrations than the control group. In addition, they demonstrated significantly lower scores in anxiety, attention problems, aggressive behavior, externalizing problems, attention deficit hyperactivity problems and oppositional defiant problems than the control group. Conclusions: Vitamin D supplementation may prevent the development of cardiometabolic risk manifestations and may improve anxiety-related, externalizing (behavioral) and internalizing (emotional) problems in overweight and obese children and adolescents with vitamin D deficiency or insufficiency.</p><p> </p>
Lorem Ipsum Press
Research Funding of the National and Kapodistrian University of Athens (UOA) Medical School, Athens, Greece
2021-01-07 15:50:51
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http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/255
Journal of Molecular Biochemistry; Vol 9, No 1 (2020)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
oai:ojs.jmolbiochem.com:article/268
2021-01-07T15:50:51Z
JmolBiochem:COMM
"201230 2020 eng "
dc
DNA replication control: Liquid-liquid phase separation comes into play
Nathanailidou, Patroula; Laboratory of General Biology, School of Medicine, University of Patras
Taraviras, Stavros
Lygerou, Zoi
DNA replication; phase separation; replication program; DNA replication licensing
<p>Liquid-liquid phase separation (LLPS) has been recently suggested as a new potential mechanism underpinning various organizational aspects of the cell, from the formation of sub-cellular, biomolecule enrichments to the assembly of organelles. In eukaryotes, DNA replication follows a strict temporal and spatial program that is majorly affected by the chromatin structure, the nuclear organization and the availability of limiting initiation factors, however the regulatory mechanisms driving the process have not been fully elucidated. Original data published lately revealed for the first time that the components of the pre-replicative complex, ORC, Cdc6 and Cdt1, are able to phase separate indicating a possible connection between LLPS and DNA replication. Here, we critically present these preliminary data and propose mechanistic models that could support this regulatory link and lead to new future research directions.</p>
Lorem Ipsum Press
State Scholarships Foundation (IKY), University of Patras
2021-01-07 15:50:51
application/pdf
http://www.jmolbiochem.com/index.php/JmolBiochem/article/view/268
Journal of Molecular Biochemistry; Vol 9, No 1 (2020)
en
Articles will be published under the terms of the Creative Commons Attribution License allowing unrestricted copying, distribution, transmission and adaptation of the work, under the condition that the original article is properly cited.More specifically, authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
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