Email the author Mannose-rich guar gum nanoparticles as a novel therapeutic drug against inflammatory diseases
Abstract
The potential to deliver nanoparticles, like polymer-based nanoparticles that can be enriched with functional groups to ensure entry into cells, directly into targeted cells is important for the therapy of inflammatory diseases. Plant-derived nanoparticles, with inherent anti-inflammatory activity and modified to allow receptor-mediated uptake, can be used as effective therapy with minimal side effects. The particle used in this study is an edible polysaccharide, derived from Cyamopsis tetragonoloba, with a galactomannan component. The particle was made mannose-rich to increase specificity towards cells expressing mannose receptors, and initially tagged with rhodamine isothiocyanate to trace its path. This study aimed to determine the therapeutic effect of the guar gum nanoparticle (GN) in vitro and in vivo in inflammatory diseases. In vitro studies on RAW 264.7 cells showed successful uptake of the nanoparticle, in a short duration of time, via their mannose receptors. Nitric oxide and MTS assays showed anti-inflammatory effects of GN. In vivo mouse model of thioglycollate-induced peritonitis showed significant decrease in inflammation, indicating its anti-inflammatory effect, and increase in clonogenic potential, indicating its regenerative potential, on intraperitoneal administration of GN. The results reflect the potential of the nanoparticle in cellular trafficking, site- specific drug delivery and bioimaging applications.
References
Ahuja M, Kumar A & Singh K 2012 Synthesis, characterization and in vitro release behavior of carboxymethyl xanthan. Int J Bio. Macromol 51 1086-1090
Akerman ME, Chan WC, Laakkonen P, Bhatia SN & Ruoslahti E 2002 Nanocrystal targeting in vivo. Proc Nat. Acad Sci USA 99 12617-12621
Brandenberger C, Mühlfeld C, Ali Z, Lenz AG, Schmid O, Parak WJ, Gehr P & Rothen-Rutishauser B 2010 Quantitative evaluation of cellular uptake and trafficking of plain and polyethylene glycol-coated gold nanoparticles. Small 6 1669-1678
Florence AT & Hussain N 2001 Transcytosis of nanoparticle and dendrimer delivery systems: evolving vistas. Adv Drug Deliv Rev 50 S69-89
Ghosh SK, Abdullah F & Mukherjee A 2015 Fabrication and fluorescent labeling of guar gum nanoparticles in a surfactant free aqueous environment. Mater Sci Eng C Mater Biol Appl 46 521-529
Hartemink R, Schoustra SE & Rombouts FM 1999 Degradation of Guar Gum by intestinal bacteria. Biosci Microfl 18 17-25
Higashi N, Fujioka K, Denda-Nagai K, Hashimoto S, Nagai S, Sato T, Fujita Y, Morikawa A, Tsuiji M, Miyata-Takeuchi M, Sano Y, Suzuki N, Yamamoto K, Matsushima K & Irimura T 2002 The macrophage C-type lectin specific for galactose/N-acetylgalactosamine is an endocytic receptor expressed on monocyte-derived immature dendritic cells. J Biol Chem 277 20686-20693
Hoet PH & Nemery B 2001 Stimulation of phagocytosis by ultrafine particles. Toxicol Appl Pharmacol 176 203
Honery S & Zahir F 2013 Effect of zeta potential on the properties of Nano drug delivery systems- a review. Trop J Pharm Res 12 255-264
Kato T, Yashiro T, Murata Y, Herbert DC, Oshikawa K, Bando M, Ohno S & Sugiyama Y 2003 Evidence that exogenous substances can be phagocytized by alveolar epithelial cells and transported into blood capillaries. Cell Tissue Res 311 47-51
Kawasaki T, Ii M, Kozutsumi Y & Yamashina I 1986 Isolation and characterization of a receptor lectin specific for galactose/N-acetylgalactosamine from macrophages. Carbohydr Res 151 197-206
Lam D, Harris D & Qin Z 2013 Inflammatory Mediator Profiling Reveals Immune Properties of Chemotactic Gradients and Macrophage Mediator Production Inhibition during Thioglycollate Elicited Peritoneal Inflammation. Med Inflam 2013 931562
Leteux C, Chai W, Loveless RW, Yuen CT, Uhlin-Hansen L, Combarnous Y, Jankovic M, Maric SC, Misulovin Z, Nussenzweig MC & Feizi T 2000 The cysteine-rich domain of the macrophage mannose receptor is a multispecific lectin that recognizes chondroitin sulfates A and B and sulfated oligosaccharides of blood group Lewis (a) and Lewis (x) types in addition to the sulfated N-glycans of lutropin. J Exp Med 191 1117-1126
Liu Y, Chirino AJ, Misulovin Z, Leteux C, Feizi T, Nussenzweig MC & Bjorkman PJ 2000 Crystal structure of the cysteine-rich domain of mannose receptor complexed with a sulfated carbohydrate ligand. J Exp Med 191 1105-1116
MacMicking J, Xie QW & Nathan C 1997 Nitric oxide and macrophage function. Annu Rev Immunol 15 323-350
McKenzie EJ, Taylor PR, Stillion RJ, Lucas AD, Harris J, Gordon S & Martinez-Pomares L 2007 Mannose Receptor Expression and Function Define a New Population of Murine Dendritic Cells. J Immunol 178 4975-4983
Mitra S, Biswas S, Sinha A, Jana NR & Banerjee ER 2015 Therapeutic use of Fisetin and Fisetin Loaded on Mesoporous Carbon Nanoparticle (MCN) in Thioglycollate-induced Peritonitis. J Nanomed Nanotechnol 6 332
Oberdorster G, Sharp Z, Atudorei V, Elder A, Gelein R, Lunts A, Kreyling W & Cox C 2002 Extrapulmonary translocation of ultrafine carbon particles following whole-body inhalation exposure of rats. J Toxicol Environ Health A 65 1531-1543
Rahman K 2007 Studies on free radicals, antioxidants, and co-factors. Clin Interv Aging 2 219-236
Sahoo R, Jacob PJS & Sahoo S 2013 Biomedical applications of green biopolymer guar gum. J Pharm Biomed Sci 35 1783-1787
Saikh T & Kumar SS 2011 Pharmaceutical and pharmacological profile of guar gum an overview. Int J Pharm Pharm Sci 3 38-40
Suzuki N, Yamamoto K, Toyoshima S, Osawa T & Irimura T 1996 Molecular cloning and expression of cDNA encoding human macrophage C-type lectin. Its unique carbohydrate binding specificity for Tn antigen. J Immunol 156 128-135
Zhang X, Goncalves R & Mosser DM 2008 The isolation and characterization of murine macrophages. Curr Protoc Immunol 14 14.1
Zhao F, Zhao Y, Liu Y, Chang X, Chen C & Zhao Y 2011 Cellular uptake, intracellular trafficking, and cytotoxicity of nanomaterials. Small 7 1322-1337
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