Identification of microRNA-365 as a negative regulator of endothelial cell proliferation

Xiao Wu, Fan Jiang

Abstract


Recent studies have shown that human microRNA miR-365 has significant inhibitory effects on proliferation of transformed cancer cells and vascular smooth muscle cells. However, the effects of miR-365 on proliferation and migration of vascular endothelial cells remain unknown. Using human umbilical vein endothelial cells and in vitro assays, we demonstrated that miR-365 was a suppressor of endothelial cell proliferation, whereas cell migration was not affected by miR-365. We also identified that the expression level of the serine/threonine protein kinase serum- and glucocorticoid-regulated kinase-1 (SGK-1) was regulated by miR-365. The cytostatic effect of miR-365 was mimicked by the specific SGK-1 inhibitor GSK 650394. We further demonstrated that microvesicles isolated from plasma of patients with intracerebral hemorrhage, in which the level of miR-365 was elevated, decreased the expression level of SGK-1, and this effect was abolished in cells pretreated with miR-365 antagomir. However, we did not observe a significant effect of the microvesicles on cell proliferation. It is suggested that miR-365 may have important roles in vascular physiology and/or pathophysiology by modulating endothelial cell proliferation.

 


Keywords


microRNA; miR-365; vascular endothelial cell; proliferation; serum- and glucocorticoid-regulated kinase-1; microvesicle

References


Bonauer A, Boon RA & Dimmeler S 2010 Vascular microRNAs. Curr Drug Targets 11 943-949

Bruhn MA, Pearson RB, Hannan RD & Sheppard KE 2010 Second AKT: the rise of SGK in cancer signalling. Growth Factors 28 394-408

Burgon J, Robertson AL, Sadiku P, Wang X, Hooper-Greenhill E, Prince LR, Walker P, Hoggett EE, Ward JR, Farrow SN, Zuercher WJ, Jeffrey P, Savage CO, Ingham PW, Hurlstone AF, Whyte MK & Renshaw SA 2014 Serum and glucocorticoid-regulated kinase 1 regulates neutrophil clearance during inflammation resolution. J Immunol 192 1796-805

Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G & Bonnerot C 2005 Exosomal-like vesicles are present in human blood plasma. Int Immunol 17 879-887

Chen Z, Huang Z, Ye Q, Ming Y, Zhang S, Zhao Y, Liu L, Wang Q & Cheng K 2015 Prognostic significance and anti-proliferation effect of microRNA-365 in hepatocellular carcinoma. Int J Clin Exp Pathol 8 1705-1711

Condorelli G, Latronico MV & Cavarretta E 2014 microRNAs in cardiovascular diseases: current knowledge and the road ahead. J Am Coll Cardiol 63 2177-2187

Datla SR, Peshavariya H, Dusting GJ, Mahadev K, Goldstein BJ & Jiang F 2007 Important role of Nox4 type NADPH oxidase in angiogenic responses in human microvascular endothelial cells in vitro. Arterioscler Thromb Vasc Biol 27 2319-2324

Fichtlscherer S, De Rosa S, Fox H, Schwietz T, Fischer A, Liebetrau C, Weber M, Hamm CW, Roxe T, Muller-Ardogan M, Bonauer A, Zeiher AM & Dimmeler S 2010 Circulating microRNAs in patients with coronary artery disease. Circ Res 107 677-684

Goren Y, Kushnir M, Zafrir B, Tabak S, Lewis BS & Amir O 2012 Serum levels of microRNAs in patients with heart failure. Eur J Heart Fail 14 147-154

Guo D, Liu J, Wang W, Hao F, Sun X, Wu X, Bu P, Zhang Y, Liu Y, Liu F, Zhang Q & Jiang F 2013 Alteration in Abundance and Compartmentalization of Inflammation-Related miRNAs in Plasma After Intracerebral Hemorrhage. Stroke 44 1739-1742

Hata A 2013 Functions of microRNAs in cardiovascular biology and disease. Annu Rev Physiol 75 69-93

Kang SM, Lee HJ & Cho JY 2013 MicroRNA-365 regulates NKX2-1, a key mediator of lung cancer. Cancer Lett 335 487-494

Kim MH, Ham O, Lee SY, Choi E, Lee CY, Park JH, Lee J, Seo HH, Seung M, Min PK & Hwang KC 2014 MicroRNA-365 inhibits the proliferation of vascular smooth muscle cells by targeting cyclin D1. J Cell Biochem 115 1752-1761

Kin K, Miyagawa S, Fukushima S, Shirakawa Y, Torikai K, Shimamura K, Daimon T, Kawahara Y, Kuratani T & Sawa Y 2012 Tissue- and plasma-specific MicroRNA signatures for atherosclerotic abdominal aortic aneurysm. J Am Heart Assoc 1 e000745

Li P, Teng F, Gao F, Zhang M, Wu J & Zhang C 2015 Identification of circulating microRNAs as potential biomarkers for detecting acute ischemic stroke. Cell Mol Neurobiol 35 433-447

Li P, Zhang Q, Wu X, Yang X, Zhang Y, Li Y & Jiang F 2014 Circulating microRNAs serve as novel biological markers for intracranial aneurysms. J Am Heart Assoc 3 e000972

Li S, Zhu J, Zhang W, Chen Y, Zhang K, Popescu LM, Ma X, Lau WB, Rong R, Yu X, Wang B, Li Y, Xiao C, Zhang M, Wang S, Yu L, Chen AF, Yang X & Cai J 2011 Signature microRNA expression profile of essential hypertension and its novel link to human cytomegalovirus infection. Circulation 124 175-184

Muller G, Schneider M, Biemer-Daub G & Wied S 2011 Microvesicles released from rat adipocytes and harboring glycosylphosphatidylinositol-anchored proteins transfer RNA stimulating lipid synthesis. Cell Signal 23 1207-1223

Nie J, Liu L, Zheng W, Chen L, Wu X, Xu Y, Du X & Han W 2012 microRNA-365, down-regulated in colon cancer, inhibits cell cycle progression and promotes apoptosis of colon cancer cells by probably targeting Cyclin D1 and Bcl-2. Carcinogenesis 33 220-225

Qin B, Xiao B, Liang D, Xia J, Li Y & Yang H 2011 MicroRNAs expression in ox-LDL treated HUVECs: MiR-365 modulates apoptosis and Bcl-2 expression. Biochem Biophys Res Commun 410 127-133

Reid G, Kirschner MB & van Zandwijk N 2010 Circulating microRNAs: Association with disease and potential use as biomarkers. Crit Rev Oncol Hematol 80 193-208

Romaine SP, Tomaszewski M, Condorelli G & Samani NJ 2015 MicroRNAs in cardiovascular disease: an introduction for clinicians. Heart 101 921-928

Roskoski R, Jr. 2004 Src protein-tyrosine kinase structure and regulation. Biochem Biophys Res Commun 324 1155-1164

Santoro MM & Nicoli S 2013 miRNAs in endothelial cell signaling: the endomiRNAs. Exp Cell Res 319 1324-1330

Thum T, Catalucci D & Bauersachs J 2008 MicroRNAs: novel regulators in cardiac development and disease. Cardiovasc Res 79 562-570

Tijsen AJ, Creemers EE, Moerland PD, de Windt LJ, van der Wal AC, Kok WE & Pinto YM 2010 MiR423-5p as a circulating biomarker for heart failure. Circ Res 106 1035-1039

Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ & Lotvall JO 2007 Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 9 654-659

Wang F, Chen C & Wang D 2014 Circulating microRNAs in cardiovascular diseases: from biomarkers to therapeutic targets. Front Med 8 404-418

Wang GK, Zhu JQ, Zhang JT, Li Q, Li Y, He J, Qin YW & Jing Q 2010 Circulating microRNA: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans. Eur Heart J 31 659-666

Webster MK, Goya L, Ge Y, Maiyar AC & Firestone GL 1993 Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum. Mol Cell Biol 13 2031-2040

Xu Z, Xiao SB, Xu P, Xie Q, Cao L, Wang D, Luo R, Zhong Y, Chen HC & Fang LR 2011 miR-365, a novel negative regulator of interleukin-6 gene expression, is cooperatively regulated by Sp1 and NF-kappaB. J Biol Chem 286 21401-21412

Zhang P, Zheng C, Ye H, Teng Y, Zheng B, Yang X & Zhang J 2014 MicroRNA-365 inhibits vascular smooth muscle cell proliferation through targeting cyclin D1. Int J Med Sci 11 765-770

Zhang Y, Liu D, Chen X, Li J, Li L, Bian Z, Sun F, Lu J, Yin Y, Cai X, Sun Q, Wang K, Ba Y, Wang Q, Wang D, Yang J, Liu P, Xu T, Yan Q, Zhang J, Zen K & Zhang CY 2010 Secreted monocytic miR-150 enhances targeted endothelial cell migration. Mol Cell 39 133-144

Zhong W, Oguljahan B, Xiao Y, Nelson J, Hernandez L, Garcia-Barrio M & Francis SC 2014 Serum and glucocorticoid-regulated kinase 1 promotes vascular smooth muscle cell proliferation via regulation of beta-catenin dynamics. Cell Signal 26 2765-2772


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