Identification of drought-induced transcription factors in peanut (Arachis hypogaea L.)

Phat M Dang, Charles Y Chen, Corley C Holbrook


Transcription factors play key roles in the regulation of genes involved in normal development as well as tolerance to biotic and abiotic stresses. Specific transcription factors that are induced in peanut under drought conditions have not been identified.  The objectives of this study were to compare gene-expression patterns of various transcription factors of a drought tolerant versus a susceptible peanut genotype under drought conditions and to identify transcripts that were regulated in a drought dependent manner. Twelve putative transcription factors were identified and real-time PCR analysis was performed which resulted in the identification of three unique transcripts in which ahERF1 was highly induced in the recovery stage; ahERF7 and ahERF8 were also highly induced by drought and returned to nominal levels after recovery.  These sequences contain DNA binding domains that are present in the APETALA2/Ethelene Responsive Factors (AP2/ERF) family of transcription factors which have been shown to be induced by stress.  Induction levels and patterns of gene-expression of ahERF1, ahERF7 and ahERF8 may be used to select plants that may have higher drought tolerance.


peanut; drought; gene-expression; PCR


Allen MD, Yamasaki K, Ohme-Takagi M, Tateno M & Suzuki M 1998 A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA. Embo J 17 5484-5496.

Araus JL, Slafer GA, Reynolds MP & Royo C 2002 Plant breeding and drought in C3 cereals: what should we breed for? Ann Bot 89 925-940.

Awal MA & Ikeda T 2002 Recovery strategy following the imposition of episodic soil moisture deficit in stands of peanut (Arachis hypogaea L.). J Agron Crop Sci 188 185-192.

Barrs HD & Weatherley PE 1962 A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aust J Biol Sci 15 413-428.

Boneh U, Biton I, Schwartz A & Ben-Ari G 2012 Characterization of the ABA signal transduction pathway in Vitis vinifera. Plant Sci 187 89-96.

Blankenship PD, Mitchell BW, Layton RC, Cole RJ & Sanders TH 1989 A low-cost microcomputer system to monitor and control an environmental control plot facility. Comput Electron Agric 4 149-155.

Chomczynski P & Sacchi N 1987 Single-step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction. Anal Biochem 162 156-159.

Girdthai T, Jogloy S, Vorasoot N, Akkasaeng C, Wongkaew S, Holbrook CC & Patanothai A 2010 Associations between physiological traits for drought tolerance and aflatoxin contamination in peanut genotypes under terminal drought. Plant Breed 129 693-699.

Guo BZ, Chen X, Hong Y, Liang X, Dang PM, Brenneman T, Holbrook CC & Culbreath AK 2009 Analysis of gene expression profiles in leaf tissues of cultivated peanuts and development of EST-SSR markers and gene discovery. Int J Plant Genomics doi: 10.1155/2009/715605

Hamidou F, Ratnakumar P, Halilou O, Mponda O, Kapewa T, Monyo E, Faye I, Ntare BR, Nigam SN, Upadhyaya HD & Vadez V 2012 Selection of intermittent drought tolerant lines across years and locations in the reference collection of groundnut (Arachis hypogaea L.). Field Crops Res 126 189-199.

Hao DY, Ohme-Takagi M & Sarai A 1998 Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant. J Biol Chem 273 26857-26861.

Harb A, Krishnan A, Ambavaram MMR & Pereira A 2010 Molecular and Physiological Analysis of Drought Stress in Arabidopsis Reveals Early Responses Leading to Acclimation in Plant Growth Plant Physiol 154 1254-1271.

Huang GT, Ma SL, Bai LP, Zhang L, Ma H, Jia P, Liu J, Zhong M & Guo ZF 2012 Signal transduction during cold, salt, and drought stresses in plants. Mol Biol Rep 39 969-987.

Hubbard KE, Siegel RS, Valerio G, Brandt B & Schroeder JI 2012 Abscisic acid and CO2 signalling via calcium sensitivity priming in guard cells, new CDPK mutant phenotypes and a method for improved resolution of stomatal stimulus-response analyses. Ann Bot 109 5-17.

Ishibashi Y, Tawaratsumida T, Kondo K, Kasa S, Sakamoto M, Aoki N, Zheng SH, Yuasa T & Iwaya-Inoue M 2012 Reactive Oxygen Species Are Involved in Gibberellin/Abscisic Acid Signaling in Barley Aleurone Cells. Plant Physiol 158 1705-1714.

Jongrungklang N, Toomsan B, Vorasoot N, Jogloy S, Boote KJ, Hoogenboom G & Patanothai A 2011 Rooting traits of peanut genotypes with different yield responses to pre-flowering drought stress. Field Crop Res 120 262-270.

Kang HG, Kim J, Kim B, Jeong H, Choi SH, Kim EK, Lee HY & Lim PO 2011 Overexpression of FTL1/DDF1, an AP2 transcription factor, enhances tolerance to cold, drought, and heat stresses in Arabidopsis thaliana. Plant Sci 180 634-641.

Kim S, Soltis PS, Wall K & Soltis DE 2006 Phylogeny and domain evolution in the APETALA2-like gene family. Mol Biol Evol 23 107-120.

Kim MJ, Lim GH, Kim ES, Ko CB, Yang KY, Jeong JA, Lee MC & Kim CS 2007 Abiotic and biotic stress tolerance in Arabidopsis overexpressing the Multiprotein bridging factor 1a (MBF1a) transcriptional coactivator gene. Biochem Biophys Res Commun 354 440-446.

Kottapalli KR, Burow MD, Burow G, Burke J & Puppala N 2007 Molecular characterization of the US peanut mini core collection using microsatellite markers. Crop Sci 47 1718-1727.

Livak KJ & Schmittgen TD 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25 402-408.

Lu C, Li Y, Chen A, Li L, Zuo J, Tian H, Luo Y & Zhu B 2010 LeERF1 improves tolerance to drought stress in tomato (Lycopersicon esculentum) and activates downstream stress-responsive genes. African J Biotechnol 9 6294-6300.

Mizoi J, Shinozaki K & Yamaguchi-Shinozaki K 2012 AP2/ERF family transcription factors in plant abiotic stress responses. Biochim Biophys Acta 1819 86-96. doi: 10.1016/j.bbagrm.2011.08.004

Moffat CS, Ingle RA, Wathugala DL, Saunders NJ, Knight H & Knight MR 2012 ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis. PLoS ONE 7 e35995.

Nautiyal PC, Ravindra V, Zala PV & Joshi YC 1999 Enhancement of yield in groundnut following the imposition of transient soil-moisture-deficit stress during the vegetative phase. Exp Agricult 35 371-385.

Pandey MK, Monyo E, Ozias-Akins P, Liang X, Guimarães P, Nigam SN, Upadhyaya HD, Janila P, Zhang X, Guo B, Cook DR, Bertioli DJ, Michelmore R & Varshney RK 2012 Advances in Arachis genomics for peanut improvement. Biotechnol Adv 30 639-651.

Puangbut D, Jogloy S, Vorasoot N, Akkasaeng C, Kesmala T, Rachaputi RCN, Wright GC & Patanothai A 2009 Association of root dry weight and transpiration efficiency of peanut genotypes under early season drought. Agricult Wat Manag 96 1460-1466.

Puangbut D, Jogloy S, Toomsan B, Vorasoot N, Akkasaeng C, Kesmala T, Rachaputi RCN, Wright GC & Patanothai A 2010 Physiological Basis for Genotypic Variation in Tolerance to and Recovery from Pre-flowering Drought in Peanut. J Agron Crop Sci 196 358-367.

Ravi K, Vadez V, Isobe S, Mir RR, Guo Y, Nigam SN, Gowda MV, Radhakrishnan T, Bertioli DJ, Knapp SJ & Varshney RK 2011 Identification of several small effect main QTLs and larg number of epistatic QTLs for drought tolerance in groundnut (Arachis hypogaea L.). Theor Appl Genet 122 1119–1132.

Rushton DL, Tripathi P, Rabara RC, Lin J, Ringler P, Boken AK, Langum TJ, Smidt L, Boomsma DD, Emme NJ, Chen X, Finer JJ, Shen QJ & Rushton PJ 2012 WRKY transcription factors: key components in abscisic acid signalling. Plant Biotechnol J 10 2-11.

Sakuma Y, Liu Q, Dubouzet JG, Abe H, Shinozaki K & Yamaguchi-Shinozaki K 2002 DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophys Res Commun 290 998-1009.

Sambrook J, Fritsch EF & Maniatis T 1989 Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press.

Stansell JR, Shepherd JL, Pallas JE, Bruce RR, Minton NA, Bell DK & Morgan LW 1976 Peanut response to soil water variable in the southeast. Peanut Sci 3 44-48

Vadez V, Rao JS, Bhatnagar-Mathur P & Sharma KK 2012 DREB1A promotes root development in deep soil layers and increases water extraction under water stress in groundnut. Plant Biol (Stuttg). doi: 10.1111/j.1438-8677.2012.00588.x

Vile D, Garnier E, Shipley B, Laurent G, Navas ML, Roumet C, Lavorel S, Díaz S, Hodgson JG, Lloret F, Midgley GF, Poorter H, Rutherford MC, Wilson PJ & Wright IJ 2005 Specific leaf area and dry matter content estimate thickness in laminar leaves. Ann Bot 96 1129-1136.

Yamaguchi-Shinozaki K & Shinozaki K 2006 Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol 57 781-803.

Zhang JH, Jia WS, Yang JC & Ismail AM 2006 Role of ABA in integrating plant responses to drought and salt stresses. Field Crop Res 97 111-119.

Zhang G, Chen M, Li L, Xu Z, Chen X, Guo J & Ma Y 2009 Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought anddiseases in transgenic tobacco. J Exp Bot 60 3781-3796.

Full Text: PDF


  • There are currently no refbacks.

Copyright © 2018 Lorem Ipsum Press